Purpose

Several clinical studies have suggested that reusable silicone hydrogel contact lens materials exhibit a two-times increased rate of corneal infiltrative events compared to reusable hydrogels. One potential factor contributing to this complication relates to the differential uptake of tear film-based pro-inflammatory cytokines. The purpose of this study was to use an in vitro assay to investigate whether four pro-inflammatory cytokines differed in their uptake onto six contemporary contact lens materials.

Methods

Conventional hydrogel (etafilcon A, omafilcon A) and silicone hydrogel (balafilcon A, comfilcon A, senofilcon A, somofilcon A) contact lens materials were soaked in solutions containing pro-inflammatory cytokines IL-1β, IL-6, IL-8 and TNF-α. Samples of the soaking solutions were collected over various time points and analyzed using the Meso Scale Discovery system, which served as a measurement of cytokine uptake onto the contact lens materials.

Results

Both conventional hydrogels (etafilcon A, omafilcon A) and two of the four silicone hydrogels tested (balafilcon A, comfilcon A), exhibited some uptake of IL-1β, IL-8 or TNF-α (p < 0.05). Senofilcon A and somofilcon A did not exhibit uptake of any of these cytokines (p > 0.05). There was no uptake of IL-6 onto any of the contact lens materials investigated (p > 0.05).

Conclusion

The contact lens materials tested did not exhibit any uptake of IL-6 and furthermore, did not exhibit more than 10 ± 3 % to 25 ± 12 % uptake of IL-1β, IL-8 or TNF-α. Numerous factors could contribute to the reported increase in corneal infiltrative events with reusable silicone hydrogel materials, however, based on these results, it appears that uptake of these four cytokines are unlikely to contribute to this finding.

中文翻译：

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泪膜炎症标志物与当代隐形眼镜材料相互作用的体外分析

目的

几项临床研究表明，与可重复使用的水凝胶相比，可重复使用的硅水凝胶隐形眼镜材料的角膜浸润事件发生率增加了两倍。导致这种并发症的一个潜在因素与基于泪膜的促炎细胞因子的不同摄取有关。本研究的目的是使用体外测定来研究四种促炎细胞因子在六种当代隐形眼镜材料上的吸收是否不同。

方法

将常规水凝胶（etafilcon A、omafilcon A）和硅水凝胶（balafilcon A、comfilcon A、senofilcon A、somofilcon A）隐形眼镜材料浸泡在含有促炎细胞因子 IL-1β、IL-6、IL-8 和 TNF 的溶液中-α。在不同的时间点收集浸泡溶液的样品，并使用 Meso Scale Discovery 系统进行分析，该系统用作隐形眼镜材料吸收细胞因子的测量。

结果

常规水凝胶（etafilcon A、omafilcon A）和测试的四种有机硅水凝胶中的两种（balafilcon A、comfilcon A）都表现出一些 IL-1β、IL-8 或 TNF-α 的吸收（p < 0.05）。Senofilcon A 和 somofilcon A 没有表现出对这些细胞因子中的任何一种的吸收（p > 0.05）。所研究的任何隐形眼镜材料均未吸收 IL-6（p > 0.05）。

结论

测试的隐形眼镜材料没有表现出任何 IL-6 吸收，此外，没有表现出超过 10±3% 至 25±12% 的 IL-1β、IL-8 或 TNF-α 吸收。许多因素可能导致报告的可重复使用硅水凝胶材料的角膜浸润事件增加，但是，基于这些结果，这四种细胞因子的摄取似乎不太可能导致这一发现。