We tested the hypothesis that concatemers of ancestral tRNAs gave rise to the 16S ribosomal RNA. We built an ancestral sequence of proto-tRNAs that showed a significant identity of 51.69% and a percentage of structural identity of 0.941 with the 3′ upper domain of 16S ribosomal molecule. We also propose a hypothesis in which the small ribosomal subunit emerged by proto-tRNA fusion and worked as a point to bind RNAs in an open structure configuration. In this context, the two ribosomal subunits initially worked independently, and that the subunit junction, with consequent primitive ribosome formation, was mediated by interactions with tRNA molecules during the primordial genetic code formation.

我们检验了祖先 tRNA 的串联体产生 16S 核糖体 RNA 的假设。我们建立了一个原始 tRNA 的祖先序列，其与 16S 核糖体分子的 3' 上域显示出 51.69% 的显着同一性和 0.941 的结构同一性百分比。我们还提出了一个假设，其中小核糖体亚基通过 proto-tRNA 融合出现，并作为一个点在开放结构配置中结合 RNA。在这种情况下，两个核糖体亚基最初独立工作，亚基连接以及随后的原始核糖体形成，是由原始遗传密码形成过程中与 tRNA 分子的相互作用介导的。