Several studies have reported that miR-885-5p was dysregulated in a variety of cancers. However, there are few studies on the biological function of miR-885-5p in gastric cancer (GC). In this study, we investigated the biological function and underlying mechanism of miR-885-5p in GC. Quantitative real-time PCR was used to examine the expression of miR-885-5p in GC. Bioinformatics analysis was used to predict the target of miR-885-5p and confirmed using the luciferase reporter assay. Wound-healing and Transwell assay were conducted to evaluate the biological function of miR-885-5p and malic enzyme 1 (ME1). Western blotting was used to assess molecular changes. Hepatic and lung metastasis models were constructed and used to verify the role of miR-885-5p. We found that the expression of miR-885-5p was significantly downregulated in GC. Overexpression of miR-885-5p inhibited invasion and metastasis of GC in vivo and in vitro, while inhibition of miR-885-5p has the opposite result in vitro. ME1 is a direct target of miR-885-5p, overexpressed in GC, associated with poor prognosis. Overexpression of miR-885-5p negatively regulates ME1 and causes changes in downstream molecules Vimentin and Fibronectin. Our research found that miR-885-5p plays a tumor suppressor gene and could potentially serve as a biomarker and therapeutic target in GC.

中文翻译：

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miR-885-5p 通过靶向苹果酸酶 1 抑制胃癌的侵袭和转移


多项研究表明 miR-885-5p 在多种癌症中失调。然而，关于miR-885-5p在胃癌（GC）中的生物学功能的研究很少。在本研究中，我们研究了 miR-885-5p 在 GC 中的生物学功能和潜在机制。采用实时定量PCR检测GC中miR-885-5p的表达。使用生物信息学分析来预测 miR-885-5p 的靶标，并使用荧光素酶报告基因测定进行确认。进行伤口愈合和Transwell实验来评估miR-885-5p和苹果酸酶1（ ME1 ）的生物学功能。蛋白质印迹用于评估分子变化。构建肝和肺转移模型并用于验证miR-885-5p的作用。我们发现 miR-885-5p 的表达在 GC 中显着下调。过表达miR-885-5p可在体内和体外抑制GC的侵袭和转移，而抑制miR-885-5p在体外则具有相反的结果。 ME1是 miR-885-5p 的直接靶标，在 GC 中过度表达，与不良预后相关。 miR-885-5p 的过度表达会负向调节ME1并导致下游分子波形蛋白和纤连蛋白发生变化。我们的研究发现 miR-885-5p 发挥抑癌基因的作用，有可能作为 GC 的生物标志物和治疗靶点。