The authors would like to clarify two elements in the paper entitled “Yes‐associated protein expression in germ cells is dispensable for spermatogenesis in mice” (1).

First, the authors realized that the Yap^flox/flox; Ddx4^cre/+ genotype used to describe the mutant animals is technically incorrect and should be changed to Yap^flox/−; Ddx4^cre/+ throughout the manuscript.

Second, Figure 1a was misinterpreted, as we suggested that the presence of the Cre‐recombined floxed allele meant that recombination had already occurred in germ cells by the time of birth. However, this statement is erroneous, as the PCR genotype analyses presented in Figure 1a cannot distinguish between newly recombined alleles in germ cells and the recombined allele coming from the male parent. Figure 1a is therefore not relevant and should be removed. The first paragraph of the “results and discussion” section should read:

“To evaluate the role of YAP in spermatogenesis, we generated a germ cell‐specific conditional knockout of Yap by crossing Yap^flox mice with the Ddx4^cre strain (Yap^flox/−; Ddx4^cre/+). The Ddx4^cre strain is known to drive Cre‐mediated recombination in germ cells starting at embryonic day e15, and reaching a high level of efficiency by the time of birth (Gallardo, Shirley, John, & Castrillon, 2007). To confirm the loss of YAP in germ cells, immunohistochemistry analyses were performed on testes from 2 month‐old mice. As previously described, YAP expression was detected in the cytoplasm and nucleus of spermatogonia and Sertoli cells of the control Yap^flox/flox mice (Levasseur et al. 2017, Figure 1a). In Yap^flox/−; Ddx4^cre/+ mice, expression of YAP remained in the Sertoli cells, but was completely lost in the spermatogonia (Figure 1b), confirming the efficiency and specificity of Yap recombination in the mutant animals.”

The corrected and figure legend would therefore be as follows:

These changes do not affect the interpretation of the other results of this study nor does it affect its conclusions.

作者想在题为“生殖细胞中的相关蛋白表达对于小鼠精子形成不可或缺”的论文中阐明两个要素（1）。

首先，作者意识到Yap ^{flox / flox} ; 用于描述突变动物的Ddx4 ^{cre / +}基因型在技术上是错误的，应该改为Yap ^{flox /-} ; 在整个手稿中为dxx4 ^{cre / +}。

其次，图1a被误解了，因为我们认为Cre重组的等位基因的存在意味着到出生时生殖细胞中已经发生了重组。但是，这种说法是错误的，因为图1a中显示的PCR基因型分析无法区分生殖细胞中新近重组的等位基因与雄性亲本的重组等位基因。因此，图1a不相关，应将其删除。“结果与讨论”部分的第一段应为：

“为了评估YAP在精子发生中的作用，我们通过将Yap ^flox小鼠与Ddx4 ^cre菌株（Yap ^{flox /-} ; Ddx4 ^{cre / +}）杂交，产生了Yap的生殖细胞特异性条件性敲除。该DDX4 ^CRE已知该菌株可在胚胎第15天开始驱动生殖细胞中Cre介导的重组，并在出生时达到高效率（Gallardo，Shirley，John和Castrillon，2007年）。为了确认生殖细胞中YAP的丢失，对2个月大小鼠的睾丸进行了免疫组织化学分析。如前所述，在Yap ^{flox / flox对照}小鼠的精原细胞和Sertoli细胞的细胞质和细胞核中检测到YAP表达（Levasseur等人，2017，图1a）。在Yap ^{flox /-中}；在Ddx4 ^{cre / +}小鼠中，YAP的表达保留在Sertoli细胞中，但在精原细胞中完全消失（图1b），从而证实了YAP的效率和特异性。突变动物的Yap重组。”

因此，更正后的图例如下：

这些变化既不影响本研究其他结果的解释，也不影响其结论。