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Enhancing oleic acid content in two commercially released peanut varieties through marker-assisted backcross breeding
Crop Science ( IF 2.0 ) Pub Date : 2021-03-23 , DOI: 10.1002/csc2.20512
Mangesh Pralhad Jadhav 1 , Malagouda D. Patil 1 , Mahesh Hampannavar 1 , Venkatesh S 1 , Pavana Dattatreya 1 , Kenta Shirasawa 2 , Janila Pasupuleti 3 , Manish K. Pandey 4 , Rajeev K. Varshney 4 , Ramesh S. Bhat 1
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Two elite cultivars of peanut (Arachis hypogaea L.), GPBD 4 and G 2–52, with high productivity, oil content, resistance to late leaf spot [Phaeoisariopsis personata (Berk. & Curt) V. Arx.] (LLS) and rust (Puccinia arachidis Speg.) diseases were improved for oleic acid content using marker-assisted backcrossing. Since both the recurrent parents already possessed the mutant allele at AhFAD2A, only mutant allele at AhFAD2B was transferred from the donor ‘SunOleic 95R’ (oleate of 80.6%). Three rounds of backcrossing with foreground selection using allele-specific polymerase chain reaction (PCR) and Kompetitive allele-specific PCR (KASP) assay identified a large number of plants homozygous for the mutant allele at AhFAD2B in BCnF2 generations. Evaluation of the advanced generations could identify six and 10 lines with significantly higher oleate than GPBD 4 and G 2–52, respectively. Considering the yield, shelling percentage, oil, and oleate content, the most promising lines HOBC2GPS_7 and HOBC2G2S_5 were selected with 112 and 142% oleate recovery over GPBD 4 and G 2–52, respectively. Double digest restriction-site-associated DNA sequencing of these superior lines showed background genome recovery of 77.5 and 69.0%, respectively. These advanced breeding lines with high oleate (∼80%), resistance to LLS and rust and high productivity are under further trials for possible release as varieties for commercial cultivation.

中文翻译:

通过标记辅助回交育种提高两个商业发布的花生品种的油酸含量

花生的两个优良品种(Arachis hypogaea L.),GPBD 4 和 G 2-52,具有高生产力、含油量、抗晚叶斑病 [ Phaeoisariopsis personata (Berk. & Curt) V. Arx.] (LLS) 和锈病 ( Puccinia arachidis Speg.) 病害的油酸含量使用标记辅助回交得到改善。由于轮回亲本均已具有AhFAD2A的突变等位基因,因此只有AhFAD2B 的突变等位基因从供体“SunOleic 95R”(80.6% 的油酸酯)转移而来。使用等位基因特异性聚合酶链反应 (PCR) 和竞争性等位基因特异性 PCR (KASP) 测定进行三轮与前景选择的回交,在 BC n F 2代中鉴定了大量纯合的植物AhFAD2B突变等位基因。对先进世代的评估可以分别鉴定出 6 和 10 株油酸明显高于 GPBD 4 和 G 2-52。综合考虑产量、脱壳率、含油量和油酸含量,最有前景的品系 HOBC 2 GPS_7 和 HOBC 2选择的 G2S_5 的油酸回收率分别为 GPBD 4 和 G 2-52 的 112% 和 142%。这些优良品系的双消化限制性位点相关 DNA 测序显示背景基因组回收率分别为 77.5% 和 69.0%。这些具有高油酸 (~80%)、抗 LLS 和锈病以及高生产力的先进育种品系正在进一步试验中,以作为商业栽培品种发布。
更新日期:2021-03-23
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