The goal of this research was to develop a new genetic database of simple sequence repetition (SSR) primers for faba and classify them according to their target genes and respective biological processes. Approximately 75,605 and 148,196 previously published genomic and transcriptomic faba sequences, respectively, have been used to detect possible SSRs. The numbers of identified SSRs from each dataset were 25,502 and 12,319, respectively. The distribution of different repeat classes indicated that trinucleotides represent the largest number of repeat counts, followed by dinucleotides. The extracted genic SSR sequences were used to design 1091 polymerase chain reaction (PCR) primers, of which only 238 (21.8%) primers target genomic sequences and the other 853 PCR primers targeted transcriptomic sequences. The annotation of gene-targeted SSRs showed that approximately 897 genes were targeted by our SSR primers. Approximately 1890 gene ontology (GO) identification codes have been obtained. The GO keywords were distributed among distinct molecular cell features. The highest redundancies involved 554 technical words, 196 domains, and 160 molecular feature phrases. These GO codes belonged to the general level of GO and included molecular function, cellular component, and biological process (544, 670, and 676 GOs, respectively). Twenty-seven SSR PCR primers were synthesized to 12 Egyptian faba bean genotypes. Approximately 11 SSR provided one to two PCR bands, whereas other SSRs provided only one sharp band with polymorphic band size. There were 13 polymorphic primers. The polymorphism information content was 0.3, which implied moderate informativeness.

本研究的目的是为蚕豆开发一个新的简单序列重复（SSR）引物遗传数据库，并根据其靶基因和各自的生物学过程对它们进行分类。分别使用大约 75,605 和 148,196 个先前发表的基因组和转录组蚕豆序列来检测可能的 SSR。从每个数据集中识别的 SSR 数量分别为 25,502 和 12,319。不同重复类别的分布表明，三核苷酸代表最多的重复计数，其次是二核苷酸。提取的基因SSR序列用于设计1091个聚合酶链反应（PCR）引物，其中只有238个（21.8%）引物靶向基因组序列，其他853个PCR引物靶向转录组序列。基因靶向 SSR 的注释表明我们的 SSR 引物靶向了大约 897 个基因。已经获得了大约1890个基因本体（GO）识别码。GO 关键字分布在不同的分子细胞特征中。最多的冗余涉及554个技术词、196个领域和160个分子特征短语。这些 GO 代码属于 GO 的一般级别，包括分子功能、细胞成分和生物过程（分别为 544、670 和 676 个 GO）。针对 12 个埃及蚕豆基因型合成了 27 个 SSR PCR 引物。大约 11 个 SSR 提供​​一到两个 PCR 条带，而其他 SSR 仅提供一个具有多态性条带大小的尖锐条带。有13个多态引物。多态信息含量为0.3，表明信息量中等。