Abstract

Fibrin is one of the promising biomaterials in tissue engineering for creating favorable conditions for the formation of a capillary-like vascular network. The use of native fibrin is limited due to its rapid degradation and opacity. To improve its properties, including biological ones, various modifications have been proposed, for example, using functionalized polyethylene glycol (PEG). In this work, homobifunctional (O,O''-bis[2-(N-succinimidyl-succinylamino)ethyl])polyethylene glycol of various molecular weights was used to modify fibrin. The modification of fibrin with vascular endothelial growth factor was also analyzed for possible stimulation of the formation of tubule-like structures. Small-angle X-ray scattering (SAXS) data and ab initio modeling of the three-dimensional particle shape showed that PEG–fibrin conjugates consist of filamentous particles, depending on the size of the PEG molecule, the molar ratio of fibrin to PEG, and the presence or absence of vascular endothelial growth factor.

中文翻译：

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小角度X射线散射分析天然纤维蛋白的结构特征及其与聚乙二醇和血管内皮生长因子的结合

摘要

纤维蛋白是组织工程中有希望的生物材料之一，可为形成毛细管状血管网络创造有利条件。天然纤维蛋白的使用由于其快速降解和不透明而受到限制。为了改善其性能，包括生物学性能，已经提出了各种修饰，例如，使用官能化的聚乙二醇（PEG）。在这项工作中，同双功能（O，O ''-bis [2-（N-各种分子量的-琥珀酰亚胺基-琥珀酰氨基）乙基]）聚乙二醇用于修饰纤维蛋白。还分析了纤维蛋白对血管内皮生长因子的修饰作用，以刺激可能的肾小管样结构的形成。小角度X射线散射（SAXS）数据和三维颗粒形状的从头计算表明，PEG-纤维蛋白结合物由丝状颗粒组成，具体取决于PEG分子的大小，纤维蛋白与PEG的摩尔比，以及是否存在血管内皮生长因子。