Pancreatic cancer has one of the worst survival rates of all major cancers, with pancreatic cystic lesions accounting for one in three pancreatic surgeries. The current gold-standard for diagnosis of pancreatic cyst malignancy is based on the endoscopic ultrasound guided fine-needle aspiration (EUS-FNA) procedure, which suffers from a low accuracy in detecting malignancy. Here we present the design and two-photon polymerization based fabrication of refractive and reflective non-contact probes, capable of rapid surveillance of the entire internal cyst surface-an advance over the contact probe we recently developed that allowed, for the first time, reliable evaluation of pancreatic cyst malignant potential in vivo. We employed a novel two-photon polymerization technique, which allows direct laser-writing to an accuracy of tens of nanometers, to fit the probe within the 540 micrometer internal diameter EUS-FNA needle. The newly constructed probes show excellent separation of the illumination and collection beams, essential for proper operation of the spatial gating method. These probes can be used clinically to perform rapid “optical biopsy”, ultimately eliminating unnecessary pancreatic surgeries on benign cysts and dangerous delays in surgical removal of malignant cysts, improving patient prognosis and quality of life.

胰腺癌是所有主要癌症中存活率最低的癌症之一，胰腺囊性病变占胰腺手术的三分之一。目前诊断胰腺囊肿恶性肿瘤的金标准是基于超声内镜引导下细针穿刺（EUS-FNA）程序，其在检测恶性肿瘤方面的准确性较低。在这里，我们展示了折射和反射非接触式探针的设计和基于双光子聚合的制造，能够快速监测整个内部囊肿表面——这是我们最近开发的接触式探针的一个进步，它第一次允许可靠评估体内胰腺囊肿恶性潜能. 我们采用了一种新颖的双光子聚合技术，该技术允许直接激光写入精度为数十纳米，以将探头安装在 540 微米内径的 EUS-FNA 针头内。新构建的探头显示出照明和收集光束的出色分离，这对于空间选通方法的正确操作至关重要。这些探头可用于临床进行快速“光学活检”，最终消除良性囊肿不必要的胰腺手术和恶性囊肿手术切除的危险延误，改善患者预后和生活质量。