There is considerable evidence to increase diosgenin and other medicinally useful compounds production in fenugreek with microbial inoculations. Fenugreek (Trigonella foenum-graecum L.), as an annual medicinal plant, is extensively cultivated in most regions of the world. In the present study we evaluate the role of plant growth-promoting rhizobacteria (PGPR) inocula (Pseudomonas putida and Bradyrhizobium japonicum) alone or in combination with an arbuscular mycorrhizal fungus(AMF) (Glomus intraradices) on diosgenin content, some biochemical characteristics and phosphorus accumulation of fenugreek plants subjected to drought stress. ACC deaminase activity was detected in both bacteria and it was 2.1 fold higher in B. japonicum than the P. putida. Results showed that diosgenin content was commonly higher in leaves under non-stress vs drought stress condition. In non-stress, plants with dual inoculation (G. intraradices and B. japonicum) resulted in highest diosgenin content significantly different with other treatments. The leaf proline content was the highest in plant subjected to drought stress with dual inoculation (G. intraradices and P. Putida), whereas the highest total soluble proteins was belonged to plants with triple inoculation(P. putida, B. japonicum and G. intraradices). Bacterial and fungal inoculation decreased peroxidase activity both in stress and non-stress condition. The highest amount of phosphorous in the roots was assigned to single inoculation with P. putida in stress and with G. intraradices in non-stress condition. The present study provides a good insight on the effect of PGPR and AMF on diosgenin content as a major bioactive constituent.

有大量证据表明，通过接种微生物可以增加胡芦巴中薯os皂苷元和其他可药用化合物的产量。胡芦巴（Trigonella foenum-graecum L.），一年生药用植物，在世界大多数地区广泛种植。在本研究中，我们评估了单独或与丛枝菌根真菌（AMF）（Glomus intraradices）结合使用的促植物根瘤菌（PGPR）接种物（Pseudomonas putida和japonicum japonicum）对薯os皂甙元含量，某些生化特性和磷的作用胡芦巴植物在干旱胁迫下的积累。在两种细菌中均检测到ACC脱氨酶活性，其活性比ACC高2.1倍。B. japonicum比恶臭假单胞菌。结果表明，在非胁迫和干旱胁迫条件下，叶片中薯gen皂苷元含量通常较高。在非胁迫条件下，双重接种的植物（G. intraradices和B. japonicum）导致薯di皂甙元含量最高，与其他处理方法明显不同。叶片脯氨酸含量在双重接种的干旱胁迫下最高（G. intraradices和P. Putida），而总可溶性蛋白最高的是三次接种的植物（P. putida，B. japonicum和G.）。内半径）。在压力和非压力条件下，细菌和真菌接种均会降低过氧化物酶活性。根系中最高的磷含量被指定为在压力下使用恶臭假单胞菌和在非胁迫条件下用G. intraradices进行单次接种。本研究对PGPR和AMF对薯os皂甙元含量（作为主要生物活性成分）的影响提供了很好的见解。