Porous microcarriers have aroused increasing attention recently by facilitating oxygen and nutrient transfer, supporting cell attachment and growth with sufficient cell seeding density. In this study, porous polyetheretherketone (PEEK) microcarriers coated with mineralized extracellular matrix (mECM), known for their chemical, mechanical and biological superiority, were developed for orthopedic applications. Porous PEEK microcarriers were derived from smooth microcarriers using a simple wet-chemistry strategy involving the reduction of carbonyl groups. This treatment simultaneously modified surface topology and chemical composition. Furthermore, the microstructure, protein absorption, cytotoxicity and bioactivity of the obtained porous microcarriers were investigated. The deposition of mECM through repeated recellularization and decellularization on the surface of porous MCs further promoted cell proliferation and osteogenic activity. Additionally, the mECM coated porous microcarriers exhibited excellent bone regeneration in a rat calvarial defect repair model in vivo, suggesting huge potential applications in bone tissue engineering.

中文翻译：

---

通过羟基化制备的多孔聚醚醚酮微载体与细胞源性矿化细胞外基质涂层一起促进细胞扩张和骨再生


多孔微载体最近引起了越来越多的关注，因为它促进氧气和营养物质的转移，以足够的细胞接种密度支持细胞附着和生长。在这项研究中，为骨科应用开发了涂有矿化细胞外基质（mECM）的多孔聚醚醚酮（PEEK）微载体，该微载体以其化学、机械和生物优势而闻名。多孔 PEEK 微载体是使用涉及羰基还原的简单湿化学策略从光滑微载体衍生而来的。这种处理同时改变了表面拓扑和化学成分。此外，还研究了所得多孔微载体的微观结构、蛋白质吸附、细胞毒性和生物活性。通过重复的再细胞化和脱细胞化，mECM在多孔MCs表面的沉积进一步促进了细胞增殖和成骨活性。此外，mECM涂层的多孔微载体在体内大鼠颅骨缺损修复模型中表现出优异的骨再生能力，这表明在骨组织工程中具有巨大的潜在应用前景。