ABSTRACT

Bacterial biofilm that formed when the Ti6Al4 V alloy was exposed to Escherichia coli, was monitored over 48 h by electrochemical impedance spectroscopy (EIS) and potentiodynamic polarization (PP) to estimate the rate of corrosion and the influence of the bacteria on this process. High-resolution scanning electron microscopy was used to examine bacterial growth, colonisation and the process of biofilm formation. Our results highlighted several critical points regarding the impact of E. coli and its use as a model for monitoring biofilm formation and the biocorrosion of this alloy. Impedance spectra revealed the formation of a compact passive film after 48-hour exposure to an aging culture of E. coli in chloride media. The formation of the biofilm influenced the resistance to corrosion. Biofilm impedance parameters that emerged over time corresponded directly to the properties of a typical exponential bacterial growth curve determined by ultraviolet-visible light spectroscopy.

摘要

当 Ti6Al4 V 合金暴露于大肠杆菌时形成的细菌生物膜，通过电化学阻抗谱 (EIS) 和动电位极化 (PP) 监测超过 48 小时，以估计腐蚀速率和细菌对该过程的影响。高分辨率扫描电子显微镜用于检查细菌生长、定植和生物膜形成过程。我们的结果强调了关于大肠杆菌的影响及其用作监测生物膜形成和这种合金的生物腐蚀的模型的几个关键点。阻抗谱显示，在氯化物介质中暴露于老化的大肠杆菌培养物 48 小时后，会形成致密的钝化膜。生物膜的形成影响耐腐蚀性。