Epigenetic marks do not change the sequence of DNA but affect gene expression in a cell-type specific manner by altering the activities of regulatory elements. Development of new molecular biology assays, sequencing technologies, and computational approaches enables us to profile the human epigenome in three-dimensional structure genome-wide. Here we describe various molecular biology techniques and bioinformatic tools that have been developed to measure the activities of regulatory elements and their chromatin interactions. Moreover, we list currently available three-dimensional epigenomic data sets that are generated in various human cell types and tissues to assist in the design and analysis of research projects.

中文翻译：

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绘制 3D 染色质结构中调控元件的分子和计算方法


表观遗传标记不会改变 DNA 序列，而是通过改变调控元件的活性，以细胞类型特定的方式影响基因表达。新分子生物学检测、测序技术和计算方法的发展使我们能够在全基因组范围内以三维结构描绘人类表观基因组。在这里，我们描述了各种分子生物学技术和生物信息学工具，这些技术和生物信息学工具已开发用于测量调节元件的活性及其染色质相互作用。此外，我们列出了当前可用的在各种人类细胞类型和组织中生成的三维表观基因组数据集，以协助研究项目的设计和分析。