Fungal polysaccharides belong to a very important class of biological macromolecules in nature, and have complex monosaccharide composition and structure. These studies on structure and biological activity of fungal polysaccharides have become one of the research hotspots of scholars at home and abroad. This study was performed in order to understand the structural characteristics and antioxidant activity of polysaccharides from Lenzites betulina (LBPs). The LBPs were deproteinized using sevag method, and further purified by DEAE cellulose-52 column and Sephadex G-100 column chromatographies, then the two refined polysaccharides were obtained and named LBPs-5 and LBPs-6. Fourier transform infrared spectrometry (FT-IR) showed that LBPs-5 and LBPs-6 are typical β-pyranose with characteristic peaks of polysaccharides. The molecular weight of the two water-soluble polysaccharides were estimated to be 3.235 × 103 Da and 6.196 × 103 Da by HPGPC, respectively. HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of LBPs-5 were mannose, glucuronic acid, glucose, and galactose in a molar ratio of 0.05:0.15:0.76:0.04. The monosaccharide compositions of LBPs-6 were mannose, glucuronic acid, and glucose, in a molar ratio of 0.04:0.17:0.79. Furthermore, the two water-soluble polysaccharides demonstrated strong scavenging effects on DPPH·, ABTS·+, ·OH and weak total reducing power, especially LBPs-6 was significantly stronger in scavenging rate than that of LBPs-5. The outcome of the study indicated that LBPs had good potential as medicine and food.

中文翻译：

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白菜Lenzites中的两种水溶性多糖的分离，结构特征和抗氧化活性

真菌多糖在自然界中是一类非常重要的生物大分子，具有复杂的单糖组成和结构。这些对真菌多糖的结构和生物学活性的研究已成为国内外学者的研究热点之一。进行这项研究是为了了解甜菜Lenzites betulina（LBPs）多糖的结构特征和抗氧化活性。用sevag法对LBPs进行脱蛋白处理，再用DEAE纤维素52柱和Sephadex G-100柱色谱进行纯化，得到两种精制多糖，分别命名为LBPs-5和LBPs-6。傅立叶变换红外光谱（FT-IR）显示LBPs-5和LBPs-6是典型的β-吡喃糖，具有多糖特征峰。通过HPGPC估计这两种水溶性多糖的分子量分别为3.235×103 Da和6.196×103 Da。带有PMP衍生化分析的HPLC表明，LBPs-5的单糖成分为甘露糖，葡萄糖醛酸，葡萄糖和半乳糖，摩尔比为0.05：0.15：0.76：0.04。LBPs-6的单糖组合物是甘露糖，葡萄糖醛酸和葡萄糖，摩尔比为0.04∶0.17∶0.79。此外，两种水溶性多糖对DPPH·，ABTS·+，·OH具有较强的清除作用，总还原能力较弱，特别是LBPs-6的清除率明显高于LBPs-5。研究结果表明，LBPs具有良好的药物和食品潜力。分别。带有PMP衍生化分析的HPLC表明，LBPs-5的单糖成分为甘露糖，葡萄糖醛酸，葡萄糖和半乳糖，摩尔比为0.05：0.15：0.76：0.04。LBPs-6的单糖组合物是甘露糖，葡萄糖醛酸和葡萄糖，摩尔比为0.04∶0.17∶0.79。此外，两种水溶性多糖对DPPH·，ABTS·+，·OH具有较强的清除作用，总还原能力较弱，特别是LBPs-6的清除率明显高于LBPs-5。研究结果表明，LBPs具有良好的药物和食品潜力。分别。带有PMP衍生化分析的HPLC表明，LBPs-5的单糖成分为甘露糖，葡萄糖醛酸，葡萄糖和半乳糖，摩尔比为0.05：0.15：0.76：0.04。LBPs-6的单糖组合物是甘露糖，葡萄糖醛酸和葡萄糖，摩尔比为0.04∶0.17∶0.79。此外，两种水溶性多糖对DPPH·，ABTS·+，·OH具有较强的清除作用，总还原能力较弱，特别是LBPs-6的清除率明显高于LBPs-5。研究结果表明，LBPs具有良好的药物和食品潜力。LBPs-6的单糖组合物是甘露糖，葡萄糖醛酸和葡萄糖，摩尔比为0.04∶0.17∶0.79。此外，两种水溶性多糖对DPPH·，ABTS·+，·OH具有较强的清除作用，总还原能力较弱，特别是LBPs-6的清除率明显高于LBPs-5。研究结果表明，LBPs具有良好的药物和食品潜力。LBPs-6的单糖组合物是甘露糖，葡萄糖醛酸和葡萄糖，摩尔比为0.04∶0.17∶0.79。此外，两种水溶性多糖对DPPH·，ABTS·+，·OH具有较强的清除作用，总还原能力较弱，特别是LBPs-6的清除率明显高于LBPs-5。研究结果表明，LBPs具有良好的药物和食品潜力。