Extreme microorganism has gained interest because of their special contribution to the environmental and industrial biotechnology, particularly psychrophilic microorganisms which can live and thrive at lower temperatures. They produce enzymes with great potential to adapt to extreme conditions. In this study, a novel psychrophilic bacterial strain designated as TaeBurcu001 grew (optimally at 5 °C) on a chemically defined medium containing dalapon (refer to as 2,2-dichloropropionic acid, 2,2-DCP) as the sole carbon source. TaeBurcu001 was isolated from Galindez Island, Antarctica. Phylogenetic analysis based on 16S rRNA gene sequence (GeneBank ID MN061637) showed that TaeBurcu001 was closely related to members of the genus Psychrobacter (99%). Comparisons of phenotypic and biochemical characteristics between the isolate and the other known Psychrobacter species showed that they are very similar. Chloride ion release was detected using colorimetric assay with maximum value recorded at 0.27 mmol/L in 30 mM of 2,2-DCP. Furthermore, the partial dehalogenase sequence of Psychrobacter TaeBurcu001 (GeneBank ID MW311072) showed a very high similarity (88%) to the previously reported 2-haloacid dehalogenase (DehE) from Rhizobium sp. (GeneBank ID CAA75671). To the best of our knowledge, this is the first study reporting on the isolation and characterisation of psychrophilic bacteria able to grow on halogenated compounds as a carbon source. Results are expected to gain new insight for further studies to uncover the full potential of these microorganisms to be applied in environmental processes and the remediation of contaminated ecosystems.

极端微生物因其对环境和工业生物技术的特殊贡献而引起了人们的兴趣，特别是可以在较低温度下生存和繁殖的嗜冷微生物。它们生产的酶具有适应极端条件的巨大潜力。在这项研究中，一种名为TaeBurcu001的新型嗜冷细菌菌株在含有 dalapon（称为 2,2-二氯丙酸，2,2-DCP）作为唯一碳源的化学成分确定的培养基上生长（最佳温度为 5°C）。TaeBurcu001分离自南极洲加林德兹岛。基于16S rRNA基因序列（GeneBank ID MN061637）的系统发育分析表明，TaeBurcu001与Psychrobacter属的成员密切相关(99%)。该分离株与其他已知的Psychrobacter物种之间的表型和生化特征的比较表明它们非常相似。使用比色法检测氯离子的释放，在 30 mM 2,2-DCP 中记录的最大值为 0.27 mmol/L。此外，Psychrobacter TaeBurcu001 (GeneBank ID MW311072)的部分脱卤酶序列与先前报道的来自根瘤菌的2-卤酸脱卤酶 (DehE) 显示出非常高的相似性 (88%)sp. （基因库 ID CAA75671）。据我们所知，这是第一项关于能够以卤化化合物作为碳源生长的嗜冷细菌的分离和表征的研究报告。预计结果将为进一步研究提供新的见解，以揭示这些微生物在环境过程中应用的全部潜力和污染生态系统的修复。