This study aims to determine both short- and long-term response of enriched anammox culture to Cu. Assessment of short-term inhibition is based both on total applied Cu concentration and potential bioavailable fractions like intracellular, surface-bound, soluble and free Cu ion. The half maximal inhibitory concentration (IC50) values for total applied, soluble, intracellular and cell-associated concentrations were determined as 4.57 mg/L, 1.97 mg/L, 0.71 mg/L, 1.11 mg/L, respectively. Correlation between the surface-bound fraction of Cu and inhibition response was weak, suggesting that Cu sorbed to biomass was not directly responsible for the effects on anammox activity. There was a disparity between the results of short- and long-term experiments in terms of inhibition threshold concentration (i.e. short-term IC50 = 4.57 mg/L vs long-term IC50 = 6.74 mg/L). Candidatus Kuenenia (59.8%) and Candidatus Brocadia (40.2%) were the two main anammox genera within the initial biomass sample. One of the most interesting finding of the study is the demonstration that a complete wash-out of C. Brocadia genus at an applied Cu concentration of 6.5 mg/L. This strongly indicates that C. Brocadia were not able to tolerate high copper concentrations and all nitrogen conversion was carried out by C. Kuenenia during the Cu exposure period.

这项研究旨在确定丰富的厌氧菌培养物对铜的短期和长期响应。短期抑制作用的评估基于总的铜浓度和潜在的生物利用度，例如细胞内，表面结合，可溶性和游离铜离子。总施加浓度，可溶性浓度，细胞内浓度和细胞相关浓度的半数最大抑制浓度（IC50）值分别确定为4.57 mg / L，1.97 mg / L，0.71 mg / L，1.11 mg / L。Cu的表面结合分数与抑制反应之间的相关性较弱，这表明吸附到生物质上的Cu并不是对厌氧氨氧化活性的直接影响。就抑制阈值浓度而言，短期和长期实验的结果之间存在差异（即，短期IC50 = 4）。初始生物量样品中的两个主要厌氧菌属是坎德尼亚假丝酵母（59.8％）和布鲁卡迪假丝酵母（40.2％）。该研究最有趣的发现之一就是证明，在施加的铜浓度为6.5 mg / L的情况下，彻底清除了布鲁氏杆菌属。这有力地表明，布罗卡迪亚菌不能耐受高浓度的铜，并且所有的氮转化都在铜暴露期间由库伊尼娅进行。