Pratylenchus coffeae is a migratory endoparasite of the root cortex and corm of Musa species and causes root lesions in coffee, sugarcane and citrus. Pratylenchus coffeae from diseased banana roots from Queensland were maintained in carrot jars for in vitro infection studies by nematophagous fungi. The aim of this work was to use a β-glucan specific fluorescent stain, calcofluor white, to study P. coffeae being trapped by two nematophagous fungal isolates Arthrobotrys musiformis and Arthrobotrys oligospora in vitro and determine whether the stain showed features of the nematode-fungal interaction that are not apparent using other observation techniques. These in vitro studies confirmed that when nematodes were stained after being trapped by these fungi, hyphae could be seen originating from the trapping device and growing within the bodies of the trapped nematodes. In addition, a putative fluorescence signal with calcofluor white was detected in the stylet and the pharyngeal region of P. coffeae suggesting the presence of β-glucan-like compound in these sites.

Pratylenchus coffeae是Musa物种的根皮层和球茎的迁移性内寄生物，可导致咖啡，甘蔗和柑橘的根部损伤。将来自昆士兰州患病香蕉根的桔梗咖啡保存在胡萝卜罐中，以进行线虫真菌的体外感染研究。这项工作的目的是使用β葡聚糖特异性荧光染色，卡尔科弗卢尔白，研究P. coffeae被困两个食线虫真菌分离孢节musiformis和少孢节丛在体外，并确定染色剂是否显示出线虫-真菌相互作用的特征，而使用其他观察技术则不明显。这些体外研究证实，当线虫被这些真菌捕获后被染色时，可以看到菌丝起源于捕获装置并在被捕获的线虫体内生长。另外，在咖啡果的探针和咽部区域中检测到带有钙荧光白的假定荧光信号，表明在这些部位存在β-葡聚糖样化合物。