We demonstrate a blood analysis routine by observing red blood cells through light and digital holographic microscopy in a microfluidic channel. With this setup a determination of red blood cell (RBC) concentration, the mean corpuscular volume (MCV), and corpuscular hemoglobin concentration mean (CHCM) is feasible. Cell count variations in between measurements differed by 2.47% with a deviation of $-0.26\times {10}^6$ μL to the reference value obtained from the Siemens ADVIA 2120i. Measured MCV values varied by 2.25% and CHCM values by 3.78% compared to the reference ADVIA measurement. Our results suggest that the combination of optical analysis with microfluidics handling provides a promising new approach to red blood cell counts.

中文翻译：

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光学研究单个红细胞，以确定细胞计数和微流控通道中细胞血红蛋白浓度

我们通过在微流体通道中通过光和数字全息显微镜观察红细胞来演示血液分析程序。通过这种设置，确定红细胞（RBC）浓度，平均红细胞体积（MCV）和红细胞血红蛋白浓度平均值（CHCM）是可行的。测量之间的细胞计数变化相差2.47％，偏差为$-0.26\times {10}^6$与从西门子ADVIA 2120i获得的参考值相比，微升。与参考ADVIA测量相比，测得的MCV值变化了2.25％，CHCM值变化了3.78％。我们的结果表明，光学分析与微流体处理相结合为红细胞计数提供了一种有希望的新方法。