Mudflat shellfish have evolved well-adapted strategies for coping with dynamic environmental fluxes and stressful conditions, including oxygen availability. The Manila clams Ruditapes philippinarum are worldwide cultured shellfish in marine intertidal zone, which usually encounter great risk of acute hypoxia exposure in coastal habitats. To reveal the effects of acute hypoxia on metabolic changes of the clams, we performed the integrated analysis of transcriptomics and metabolomics to investigate the global changes of genes and metabolites during acute hypoxia stress at the whole-organism level. The comparative transcriptome analysis reveals that the clams show the remarkable depression in a variety of biological performance, such as metabolic rates, neuronal activity, biomineralization activity, and cell proliferation and differentiation at the hypoxic condition. The metabolomic analysis reveals that amino acid metabolism plays a critical role in the metabolic changes of the clams in response to acute hypoxia. A variety of free amino acids may not only be served as the potential osmolytes for osmotic regulation, but also may contribute to energy production during the acute hypoxia exposure. The metabolite analysis also reveals several important biomarkers for metabolic changes, and provides new insights into how clams deal with acute hypoxia. These findings suggest that clams may get through acute hypoxia stress by the adaptive metabolic strategy to survive short-period of acute hypoxia which is likely to occur in their typical habitat. The present findings will not only shed lights on the molecular and metabolic mechanisms of adaptive strategies under stressful conditions, but also provide the signaling metabolites to assess the physiological states of clams in aquaculture.

滩涂贝类已经进化出适应性强的策略，可以应对动态环境通量和压力条件，包括氧气供应。马尼拉蛤仔菲律宾蛤仔是在海洋潮间带世界范围内养殖的贝类，通常在沿海生境中面临急性缺氧的巨大风险。为了揭示急性缺氧对蛤类代谢变化的影响，我们进行了转录组学和代谢组学的综合分析，以研究全有机体急性缺氧胁迫期间基因和代谢物的整体变化。对比转录组分析显示，蛤show在多种生物学表现中表现出显着的衰退，例如在低氧条件下的代谢率，神经元活性，生物矿化活性以及细胞增殖和分化。代谢组学分析表明，氨基酸代谢在响应急性低氧的蛤the的代谢变化中起关键作用。各种游离氨基酸不仅可以用作潜在的渗透调节渗透剂，而且还可以在急性缺氧暴露期间促进能量产生。代谢物分析还揭示了代谢变化的几个重要生物标志物，并为蛤如何处理急性缺氧提供了新见识。这些发现表明，蛤可能通过适应性代谢策略克服急性低氧应激，从而在其典型栖息地可能发生的急性低氧短期内存活下来。目前的发现不仅将阐明在压力条件下适应策略的分子和代谢机制，而且还将提供信号代谢产物以评估水产养殖中蛤的生理状态。但在急性低氧暴露期间也可能有助于产生能量。代谢物分析还揭示了代谢变化的几个重要生物标志物，并为蛤如何处理急性缺氧提供了新见识。这些发现表明，蛤可能通过适应性代谢策略克服急性低氧应激，从而在其典型栖息地可能发生的急性低氧短期内存活下来。目前的发现不仅将阐明在压力条件下适应策略的分子和代谢机制，而且还将提供信号代谢产物以评估水产养殖中蛤的生理状态。但在急性低氧暴露期间也可能有助于产生能量。代谢物分析还揭示了代谢变化的几个重要生物标志物，并为蛤如何处理急性缺氧提供了新见识。这些发现表明，蛤可能通过适应性代谢策略克服急性低氧应激，从而在其典型栖息地可能发生的急性低氧短期内存活下来。本研究结果不仅将阐明在压力条件下适应策略的分子和代谢机制，而且还将提供信号代谢产物来评估水产养殖蛤lam的生理状态。并为蛤如何处理急性缺氧提供新的见解。这些发现表明，蛤可能通过适应性代谢策略克服急性低氧应激，从而在其典型栖息地可能发生的急性低氧短期内存活下来。目前的发现不仅将阐明在压力条件下适应策略的分子和代谢机制，而且还将提供信号代谢产物以评估水产养殖中蛤的生理状态。并为蛤如何处理急性缺氧提供新的见解。这些发现表明，蛤可能通过适应性代谢策略克服急性低氧应激，从而在其典型栖息地可能发生的急性低氧短期内存活下来。目前的发现不仅将阐明在压力条件下适应策略的分子和代谢机制，而且还将提供信号代谢产物以评估水产养殖中蛤的生理状态。