In this study, the effect of calcification on methanogenic activity, basic properties, microbial community structure, and microstructure of anaerobic granular sludge (AnGS) was investigated. Results indicated that inorganic crystals were deposited in the sludge in the form of calcite, which increased sludge density and led them to deposit at the bottom of the anaerobic reactor. Calcification markedly reduced the values of volatile suspended solids (VSS) / total suspended solids (TSS) and extracellular polymeric substances (EPS) content by 35.0 % and 27.1 %, respectively. Chloroflexi, Actinobacteria, Firmicutes, and Bacteroidetes were the most dominant functional species in both types of samples, accounting for 69.1 % of the calcified samples and lower than 71.8 % of the uncalcified samples. Compared to uncalcified sludge, the abundance of Methanosaeta (9.7 %) in the calcified sludge decreased by 34.0 %. And calcification reduces the abundance of gene pathway and methane-related enzymes. In addition, the proportion of inorganic crystals in calcified sludge was 180.6 % higher than that in uncalcified sludge, while the proportion of biomass and pores decreased by 11.7 % and 73.8 %, respectively. And the proportion of larger pores was significantly reduced. These factors lead to the cumulative methane production in calcified AnGS decreased by 26.8 %, and the specific methanogenic activity (SMA) reduced by 16.2 %.

在这项研究中，研究了钙化对厌氧颗粒污泥（AnGS）产甲烷活性，基本特性，微生物群落结构和微观结构的影响。结果表明，无机晶体以方解石的形式沉积在污泥中，这增加了污泥的密度并使它们沉积在厌氧反应器的底部。钙化显着降低了挥发性悬浮固体（VSS）/总悬浮固体（TSS）和细胞外聚合物（EPS）含量的值，分别降低了35.0％和27.1％。在这两种类型的样品中，绿弯曲菌，放线菌，硬毛菌和拟杆菌属是最主要的功能性物种，占钙化样品的69.1％，未钙化样品的含量低于71.8％。与未钙化的污泥相比，钙化污泥中的甲烷菌（9.7％）减少了34.0％。钙化减少了基因途径和甲烷相关酶的丰度。此外，钙化污泥中无机晶体的比例比未钙化污泥中的高180.6％，而生物质和孔隙的比例分别降低了11.7％和73.8％。并且大孔的比例显着减少。这些因素导致钙化AnGS中的累积甲烷产量降低了26.8％，比甲烷生成活性（SMA）降低了16.2％。