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Interaction of leachable model compounds and their impact on Chinese hamster ovary cell cultivation
Biotechnology Progress ( IF 2.5 ) Pub Date : 2021-03-26 , DOI: 10.1002/btpr.3150 Dana Budde 1, 2 , Gian-Luca Albano 1 , Thomas Noll 2 , Elke Jurkiewicz 1
Biotechnology Progress ( IF 2.5 ) Pub Date : 2021-03-26 , DOI: 10.1002/btpr.3150 Dana Budde 1, 2 , Gian-Luca Albano 1 , Thomas Noll 2 , Elke Jurkiewicz 1
Affiliation
The presence of leachables in biopharmaceutical processes using single-use technologies (SUT) is well known. For the detection and quantification of the latter, extractable studies of SUT are very common nowadays. Although a mixture of compounds is regularly found in extractable studies, research has only been carried out regarding the effect of individual compounds on cell culture and the cumulative effect of a mix of leachables has not been investigated yet. In this study, a set of leachable model compounds (LMCs) was chosen and the effect of the LMCs on a Chinese hamster ovary DG44 cell line producing an IgG antibody was investigated concerning cell growth, cell cycle distribution and productivity. It was shown that even if worst-case concentrations were used, the LMCs solely impact cell growth. Additionally, interaction studies revealed that the inhibiting effect of the mix is lower than the expected cumulative effect. A strong antagonism between the antioxidant butylated hydroxytoluene and the plasticizer Tris(2-ethylhexyl)trimellitate was found using an isobologram analysis.
更新日期：2021-03-26
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