The deteriorating effects of Lead (Pb) on central nervous system (CNS) such as cerebellum has been demonstrated in previous studies. Glycoconjugates with the important role in CNS development may be affected by Pb-exposure. Utilization of antioxidant agents and herbal plants has attracted a great deal of attention on attenuating neurotoxicants-induced damage. Thus, in this study the neuroprotective effects of vitamin C and garlic on content of glycoconjugates of cerebellar cortex in Pb-exposed animals were investigated. Wistar pregnant rats were divided into: control (C), Pb-exposed (Pb) (1500 ppm lead acetate in drinking water), Pb plus vitamin C (Pb + Vit C) (500 mg/kg) intraperitoneally, Pb plus garlic (Pb + G) (1 mL /100 g body weight fresh garlic juice via gavage), Pb plus vitamin C and garlic (Pb + Vit C + G), and sham groups (Sh). Finally, levels of Pb in blood were measured in both rats and offspring on postnatal day 50 (PND50). Also, the cerebellums were removed for measuring Pb-levels and performing lectin histochemistry. Blood and cerebellar Pb-levels were increased in Pb-exposed group compared to control group (P < 0.001), whereas they were decreased significantly in Pb + Vit C, Pb + G, and Pb + Vit C + G groups (P < 0.01). By using MPA, UEA-1, and WGA lectin histochemistry, Pb-exposed group showed weak staining intensity compared to other groups. Besides, significant decrease was observed in the density of lectin-positive neurons of Pb-exposed group compared to the control group (P < 0.001). Moreover, strong staining intensity and high lectin-positive neurons were found in Pb + Vit C, Pb + G and Pb + Vit C + G groups than Pb-exposed group (P < 0.001). The present study revealed that Pb-exposure can result in alteration in the cerebellar glycoconjugates contents and co-administration of vitamin C and garlic could attenuate the adverse effects of Pb. The findings of this study revealed the ameliorating effects of vitamin C and garlic against Pb, suggesting the potential use of vitamin C and garlic as preventive agents in Pb poisoning.

中文翻译：

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维生素C和大蒜对铅暴露大鼠后代小脑皮质糖复合物变化的神经保护作用


先前的研究已证明铅 (Pb) 对小脑等中枢神经系统 (CNS) 的恶化影响。在中枢神经系统发育中起重要作用的糖缀合物可能会受到铅暴露的影响。抗氧化剂和草药的利用在减轻神经毒物引起的损伤方面引起了广泛关注。因此，在本研究中，研究了维生素 C 和大蒜对铅暴露动物小脑皮质糖复合物含量的神经保护作用。 Wistar怀孕大鼠分为：对照（C）、铅暴露（Pb）（饮用水中1500 ppm醋酸铅）、铅加维生素C（Pb + Vit C）（500 mg/kg）腹腔注射、铅加大蒜（ Pb + G）（1 mL/100 g 体重新鲜大蒜汁，灌胃），Pb 加维生素 C 和大蒜（Pb + 维生素 C + G），以及假手术组（Sh）。最后，在出生后第 50 天（PND50）测量大鼠和后代的血液中 Pb 水平。此外，还切除小脑以测量铅水平并进行凝集素组织化学。与对照组相比，Pb 暴露组的血液和小脑 Pb 水平升高 (P < 0.001)，而 Pb + Vit C、Pb + G 和 Pb + Vit C + G 组则显着降低 (P % 3C 0.01）。通过使用 MPA、UEA-1 和 WGA 凝集素组织化学，Pb 暴露组与其他组相比显示出较弱的染色强度。此外，与对照组相比，Pb暴露组凝集素阳性神经元密度显着降低（P<<0.001）。此外，Pb + Vit C、Pb + G 和 Pb + Vit C + G 组比 Pb 暴露组染色强度强，凝集素阳性神经元高（P < 0.001）。 目前的研究表明，铅暴露会导致小脑糖复合物含量的改变，同时服用维生素 C 和大蒜可以减轻铅的不利影响。这项研究的结果揭示了维生素 C 和大蒜对铅的改善作用，表明维生素 C 和大蒜可能用作铅中毒的预防剂。