Micron/nano scale topographic modification has been a significant focus of interest in current titanium (Ti) surface design. However, the influence of micron/nano structured surface on cell or bacterium behavior on the Ti implant has rarely been systematically evaluated. Moreover, except for popular microgrooves, little work has been carried out on the reaction of cells to the bionic structure. In this study, several micro-pillars mimicking cell morphology were prepared on Ti surfaces by lithography and contact printing (ICP) method, and they were further decorated with nanotube arrays by anodization technology. These surface modifications remarkablly increased the surface roughness of pristine Ti surface from 91.17 nm ± 5.57 nm to be more than 1000 nm, and reduced their water contact angles from 68.3° ± 0.7° to be 16.9° ± 2.4°. Then, the effects of these hierarchical micron/nano scale patterns on the behaviors of MG63 osteoblasts, L929 fibroblasts, SCC epithelial cells and P. gingivalis were studied, aiming to evaluate their performance in osseointegration, gingival epithelial sealing and antibacterial ability. Through an innovative scoring strategy, our findings showed that square micro-pillars with 6 µm width and 2 µm height combined with 85 nm diameter nanotubes was suitable for implant neck design, while square micro-pillars with 3 µm width and 3.6 µm height combined with 55 nm diameter nanotubes was the best for implant body design. Our study reveals the synergistic effect of the hierarchical micron/nano scale patterns on MG63 osteoblasts, L929 fibroblasts, SCC epithelial cells and P. gingivalis functions. It provides insight into the design of biomedical implant surfaces.

微米/纳米尺度的地形修改已成为当前钛（Ti）表面设计的重要关注点。然而，很少有系统地评估微米/纳米结构表面对钛植入物的细胞或细菌行为的影响。此外，除了流行的微槽以外，在细胞与仿生结构反应方面的工作很少。在这项研究中，通过光刻和接触印刷（ICP）方法在Ti表面制备了一些模拟细胞形态的微柱，并通过阳极氧化技术进一步用纳米管阵列装饰了它们。这些表面改性显着地将原始Ti表面的表面粗糙度从91.17 nm±5.57 nm增加到大于1000 nm，并将它们的水接触角从68.3°±0.7°减小到16.9°±2.4°。然后，对牙龈卟啉单胞菌进行了研究，旨在评估其在骨整合，牙龈上皮封闭和抗菌能力方面的表现。通过创新的评分策略，我们的研究结果表明，宽度为6 µm，高度为2 µm的方形微柱与直径为85 nm的纳米管相结合，适用于种植体颈部设计，而宽度为3 µm和高度为3.6 µm的方形微柱与纳米管相结合。直径为55 nm的纳米管最适合植入体设计。我们的研究揭示了微米/纳米分级模式对MG63成骨细胞，L929成纤维细胞，SCC上皮细胞和牙龈卟啉单胞菌功能的协同作用。它提供了对生物医学植入物表面设计的了解。