The full potential of managing microbial communities to support plant health is yet-unrealized, in part because it remains difficult to ascertain which members are most important for the plant. However, microbes that consistently associate with a plant species across varied field conditions and over plant development likely engage with the host or host environment. Here, we applied abundance-occupancy concepts from macroecology to quantify the core membership of bacterial/archaeal and fungal communities in the rhizosphere of the common bean (Phaseolus vulgaris). Our study investigated the microbiome membership that persisted over multiple dimensions important for plant agriculture, including major U.S. growing regions (Michigan, Nebraska, Colorado, and Washington), plant development, annual plantings, and divergent genotypes, and also included re-analysis of public data from beans grown in Colombia. We found 48 core bacterial taxa that were consistently detected in all samples, inclusive of all datasets and dimensions. This suggests reliable enrichment of these taxa to the plant environment and time-independence of their association with the plant. More generally, the breadth of ecologically important dimensions included in this work (space, time, host genotype, and management) provides an example of how to systematically identify the most stably-associated microbiome members, and can be applied to other hosts or systems.

管理微生物群落以支持植物健康的全部潜力尚未实现，部分原因是仍然难以确定哪些成员对植物最重要。然而，在不同的田间条件和植物发育过程中始终与植物物种相关联的微生物可能与宿主或宿主环境相互作用。在这里，我们应用宏观生态学中的丰度占用概念来量化普通豆 ( Phaseolus vulgaris ) 根际细菌/古细菌和真菌群落的核心成员). 我们的研究调查了在植物农业重要的多个维度上持续存在的微生物组成员，包括美国主要种植区（密歇根州、内布拉斯加州、科罗拉多州和华盛顿州）、植物发育、年度种植和不同的基因型，还包括对公共数据的重新分析来自哥伦比亚种植的豆类的数据。我们发现了 48 个核心细菌类群，它们在所有样本中都被一致检测到，包括所有数据集和维度。这表明这些分类群在植物环境中的可靠富集以及它们与植物的关联具有时间独立性。更一般地说，这项工作中包含的生态重要维度的广度（空间、时间、宿主基因型和管理）提供了一个示例，说明如何系统地识别最稳定相关的微生物组成员，