The effect of trehalose, antioxidants, and acetate buffer concentration on oxytocin stability,Journal of Peptide Science

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The effect of trehalose, antioxidants, and acetate buffer concentration on oxytocin stability
Journal of Peptide Science ( IF 1.8 ) Pub Date : 2021-03-25 , DOI: 10.1002/psc.3324
Mostafa Ghasemisarabbadieh _{1,

2,

3} , Sveinbjörn Gizurarson _{1,

3,

4} , Benjamín Ragnar Sveinbjörnsson _{2,

3}

Affiliation

Oxytocin is a cyclic nonapeptide used to induce labor and prevent bleeding after childbirth. Due to its instability, storage and transport of oxytocin formulations can be problematic in hot/tropical climates. The aim of this study was to investigate the effect of trehalose and select antioxidants (uric acid, butylated hydroxytoluene, and l-ascorbic acid) on oxytocin stability in solution. The effect of buffer composition and acetate buffer concentration was also studied. Acetate buffer was found to work better than citrate/phosphate buffer for the oxytocin stability. Lower acetate buffer concentrations (0.025 M or less) were also found to yield improved oxytocin stability compared to higher concentrations. Although known degradation pathways of oxytocin include oxidation, the antioxidants uric acid and butylated hydroxytoluene had negligible effect on the oxytocin stability while l-ascorbic acid led to significantly faster degradation. Despite trehalose's reputation as a great stabilizer for biomolecules, it also had small to negligible effect on oxytocin stability at concentrations up to 1 M in acetate buffer. These results were surprising given the present literature on trehalose as a stabilizer for various biomolecules, including proteins and lipids.

中文翻译：

海藻糖、抗氧化剂和醋酸盐缓冲液浓度对催产素稳定性的影响

催产素是一种环状九肽，用于引产和防止分娩后出血。由于其不稳定性，催产素制剂的储存和运输在炎热/热带气候中可能存在问题。本研究的目的是研究海藻糖的作用并选择抗氧化剂（尿酸、丁基化羟基甲苯和l-抗坏血酸）对溶液中催产素稳定性的影响。还研究了缓冲液组成和醋酸盐缓冲液浓度的影响。发现醋酸盐缓冲液在催产素稳定性方面比柠檬酸盐/磷酸盐缓冲液效果更好。与较高浓度相比，还发现较低的乙酸盐缓冲液浓度（0.025 M 或更低）可产生改善的催产素稳定性。虽然已知的催产素降解途径包括氧化，但抗氧化剂尿酸和丁基化羟基甲苯对催产素稳定性的影响可以忽略不计，而l-抗坏血酸导致显着更快的降解。尽管海藻糖作为生物分子的重要稳定剂享有盛誉，但在醋酸盐缓冲液中浓度高达 1 M 时，它对催产素稳定性的影响也很小甚至可以忽略不计。鉴于目前关于海藻糖作为各种生物分子（包括蛋白质和脂质）的稳定剂的文献，这些结果令人惊讶。

更新日期：2021-03-25

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