Photoacoustic microscopy is an in vivo imaging technology based on the photoacoustic effect. It is widely used in various biomedical studies because it can provide high-resolution images while being label-free, safe, and harmless to biological tissue. Polygon-scanning is an effective scanning method in photoacoustic microscopy that can realize fast imaging of biological tissue with a large field of view. However, in polygon-scanning, fluctuations of the rotating motor speed and the geometric error of the rotating mirror cause image distortions, which seriously affect the photoacoustic-microscopy imaging quality. To improve the image quality of photoacoustic microscopy using polygon-scanning, an image correction method is proposed based on accurate ultrasound positioning. In this method, the photoacoustic and ultrasound imaging data of the sample are simultaneously obtained, and the angle information of each mirror used in the polygon-scanning is extracted from the ultrasonic data to correct the photoacoustic images. Experimental results show that the proposed method can significantly reduce image distortions in photoacoustic microscopy, with the image dislocation offset decreasing from 24.774 to 10.365 μm.

中文翻译：

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基于准确的超声定位恢复光声图像

光声显微镜技术是一种基于光声效应的体内成像技术。它可以在不加标签，安全且对生物组织无害的同时提供高分辨率图像，因此在各种生物医学研究中得到了广泛的应用。多边形扫描是光声显微镜中一种有效的扫描方法，可以实现大视野范围内生物组织的快速成像。然而，在多边形扫描中，旋转的电动机速度的波动和旋转镜的几何误差引起图像失真，这严重影响了光声显微镜的成像质量。为了提高利用多边形扫描的光声显微镜的图像质量，提出了一种基于精确超声定位的图像校正方法。用这种方法 同时获得样品的光声和超声成像数据，并从超声数据中提取在多边形扫描中使用的每个反射镜的角度信息，以校正光声图像。实验结果表明，该方法可以显着减少光声显微镜中的图像畸变，图像位错偏移从24.774减小到10.365μm。