Abstract

Byssuses, which are proteinaceous fibers secreted by mollusks, are remarkable underwater adhesives. Although mussel adhesives are well known, much less is known about the byssal proteins of pearl oysters especially in the adhesive regions. In this study, adhesive proteins from the pearl oyster Pinctada fucata were studied in depth by transcriptomics and proteomics approaches. In total, 16 novel proteins were identified including a von Willebrand factor type A domain-containing protein, a thrombospondin-1-like protein, tyrosinase, mucin-like proteins, protease inhibitors, and Pinctada unannotated foot protein 3 (PUF3) to PUF6. Interestingly, PUF3-6 are enriched with glycine, serine, and PXG (X = F/Y/W/K/L) motifs and are highly expressed in the foot. The identification of byssal proteins of the pearl oyster is a key step for understanding byssus formation and may inspire the synthesis of novel adhesives for underwater use and the development of anti-biofouling strategies.

摘要

苯酚是软体动物分泌的蛋白质纤维，是出色的水下粘合剂。尽管贻贝胶粘剂是众所周知的，但对珍珠牡蛎的底蛋白尤其是在胶粘剂区域了解得很少。在这项研究中，通过转录组学和蛋白质组学方法对珍珠牡蛎Pinctada fucata的黏附蛋白进行了深入研究。总共鉴定出16种新蛋白，包括von Willebrand因子A型结构域蛋白，血小板反应蛋白1样蛋白，酪氨酸酶，粘蛋白样蛋白，蛋白酶抑制剂和Pinctada。未注释的脚蛋白3（PUF3）至PUF6。有趣的是，PUF3-6富含甘氨酸，丝氨酸和PXG（X = F / Y / W / K / L）基序，并在脚中高表达。鉴定珍珠贝的海底蛋白质是了解海底形成的关键步骤，可能会启发水下合成新型胶粘剂的合成以及抗生物污损策略的发展。