Mesoporous silica is widely used because of its unique and excellent properties, especially it can be used as a drug carrier and gene carrier in the biomedical field. After the mesoporous silica is put into clinical use, it is more likely to be exposed in human body. Therefore, the effect of mesoporous silica on human body cannot be ignored. The injury of vascular endothelial cells is a prerequisite for the occurrence of many cardiovascular diseases. As a drug and gene carrier, mesoporous silica increases its contact with vascular endothelial cells, so its toxic effect on cardiovascular system cannot be ignored. In this study, amino (NH₂) and carboxyl (COOH) were modified on mesoporous silica SBA-15 by post-grafting. The results showed that it still maintained the one-dimensional hexagonal mesoporous structure of SBA-15 and had typical mesoporous structure. Then human umbilical vein endothelial cells (HUVECs) were infected with SBA-15, NH₂-SBA-15, and COOH-SBA-15. The results showed that the functionalized mesoporous silica SBA-15 had cytotoxicity to HUVECs and damaged the cell membrane, but compared with the unmodified mesoporous silica SBA-15 the cytotoxicity of functionalized mesoporous silica SBA-15 was lower and the toxicity of carboxyl modified group was the lowest. By comparing the cell inhibition rate and the expression level of lactate dehydrogenate and reactive oxygen species induced by the three materials, oxidative damage and cell membrane damage may be two mechanisms of cytotoxicity. Mesoporous silica SBA-15 has an effect on cardiovascular system by inducing the high expression of nitric oxide, intercellular adhesive molecule-1 and vascular cell adhesive molecule-1 in HUVECs. In summary, our results show that mesoporous silica is toxic to vascular endothelial cells.

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氨基和羧基修饰的介孔二氧化硅对血管内皮细胞的细胞毒性

介孔二氧化硅因其独特和优异的性能而被广泛应用，特别是在生物医学领域可作为药物载体和基因载体。介孔二氧化硅投入临床使用后，更容易暴露于人体内。因此，介孔二氧化硅对人体的影响不容忽视。血管内皮细胞的损伤是许多心血管疾病发生的先决条件。介孔二氧化硅作为药物和基因载体，增加了与血管内皮细胞的接触，对心血管系统的毒性作用不容忽视。在本研究中，氨基 (  NH ₂ ) 和羧基 ( COOH) 通过后接枝在介孔二氧化硅 SBA-15 上进行改性。结果表明，它仍然保持了SBA-15的一维六方介孔结构，具有典型的介孔结构。然后用 SBA-15、NH ₂感染人脐静脉内皮细胞 (HUVEC)-SBA-15 和 COOH-SBA-15。结果表明，功能化介孔二氧化硅SBA-15对HUVECs具有细胞毒性并损伤细胞膜，但与未修饰的介孔二氧化硅SBA-15相比，功能化介孔二氧化硅SBA-15的细胞毒性较低，羧基修饰基团的毒性较小。最低的。通过比较三种材料诱导的细胞抑制率和乳酸脱氢盐和活性氧的表达水平，氧化损伤和细胞膜损伤可能是细胞毒性的两种机制。介孔二氧化硅 SBA-15 通过诱导 HUVEC 中一氧化氮、细胞间粘附分子-1 和血管细胞粘附分子-1 的高表达而对心血管系统产生影响。总之，我们的结果表明介孔二氧化硅对血管内皮细胞是有毒的。