ABSTRACT

In this study, a monoclonal antibody (mAb) against chlortetracycline was prepared by immunisation using chlortetracycline conjugated to bovine serum albumin (CTC-BSA) and cell fusion. One monoclonal antibody (1-2B) against chlortetracycline was obtained with an IC₅₀ of 0.33 ng/ml and a detection limit of 0.1 ng/ml. The cross reactivity of this antibody with structurally related and non-related compounds was less than 0.1%, demonstrating that the obtained mAb provided high specificity. Under optimal conditions for ic-ELISA, a linearity was obtained between 0.06 and 3.9 ng/ml of chlortetracycline. The quantification of CTC-spiked milk and honey samples was also performed, demonstrating recovery rates ranging from 95.2% to 105.6% and the coefficient of variation below 10% for both intra- and inter-variation assays. Based on the sensitivity and specificity measurement, mAb 1-2B could feasibility be used as a screening tool for CTC residue detection in milk and honey samples.

摘要

在这项研究中，通过使用与牛血清白蛋白（CTC-BSA）偶联的金霉素进行免疫接种和细胞融合来制备针对金霉素的单克隆抗体（mAb）。使用IC ₅₀获得了一种针对金霉素的单克隆抗体（1-2B）0.33 ng / ml的检出限和0.1 ng / ml的检出限。该抗体与结构相关和非相关化合物的交叉反应性小于0.1％，表明所获得的mAb提供了高特异性。在ic-ELISA的最佳条件下，金霉素的线性在0.06至3.9 ng / ml之间。还进行了四氯化碳加标的牛奶和蜂蜜样品的定量分析，结果表明，变异内和变异分析的回收率在95.2％至105.6％之间，变异系数在10％以下。基于敏感性和特异性的测量，mAb 1-2B可以用作牛奶和蜂蜜样品中CTC残留检测的筛选工具。