Monitoring the concentration of antibiotics in body fluids is essential to optimizing the therapy and minimizing the risk of bacteria resistance, which can be made with electrochemical sensors tailored with appropriate materials. In this paper, we report on sensors made with screen-printed electrodes (SPE) coated with fullerene (C60), reduced graphene oxide (rGO) and Nafion (NF) (C60-rGO-NF/SPE) to determine the antibiotic metronidazole (MTZ). Under optimized conditions, the C60-rGO-NF/SPE sensor exhibited a linear response in square wave voltammetry for MTZ concentrations from 2.5 × 10⁻⁷ to 34 × 10⁻⁶ mol/L, with a detection limit of 2.1 × 10⁻⁷ mol/L. This sensor was also capable of detecting MTZ in serum and urine, with recovery between 94% and 100%, which are similar to those of the standard chromatographic method (HPLC-UV). Because the C60-rGO-NF/SPE sensor is amenable to mass production and allows for MTZ determination with simple principles of detection, it fulfills the requirements of therapeutic drug monitoring programs.

监测体液中抗生素的浓度对于优化治疗和最大程度地降低细菌耐药性风险至关重要，这可以通过采用适当材料定制的电化学传感器来制成。在本文中，我们报告了采用涂有富勒烯 (C60)、还原石墨烯氧化物 (rGO) 和 Nafion (NF) (C60-rGO-NF/SPE) 的丝网印刷电极 (SPE) 制成的传感器，用于测定抗生素甲硝唑 ( MTZ）。^{在优化条件下，C60-rGO-NF/SPE传感器对2.5 × 10 -7}至34 × 10 ^-6 mol/L的MTZ浓度在方波伏安法中表现出线性响应 ，检测限为2.1 × 10 ^-7 摩尔/升。该传感器还能够检测血清和尿液中的MTZ，回收率在94%至100%之间，与标准色谱方法（HPLC-UV）相似。由于 C60-rGO-NF/SPE 传感器适合大规模生产，并且可以通过简单的检测原理进行 MTZ 测定，因此它满足治疗药物监测项目的要求。