Global requirement for algal foods is increasing, as they are progressively consumed for its nutrition and health. Macroalgae is a proven source of metabolites, proteins, pigments, bioactive compounds, and algal polysaccharides. The unique polysaccharides such as agar, carrageenan, porphyran, alginate, fucoidan, laminarin, and ulvan are known for its wide range of bioactivities and extensively used for applications from tissue engineering to drug delivery. However, there are few limitations due to its high molecular size, low compatibility, and hydrocolloid nature. Hence, the enzymatically produced algal oligosaccharides have drawn tremendous attention due to its green synthesis, solubility, and lower molecular size. They are reported to have bioactivities including antioxidant, antiglycemic, immunostimulatory, anti-inflammatory, and prebiotic activities, which can be used in the healthcare and nutraceutical industry for the manufacture of functional foods and dietary supplements. However, identification of potential microorganisms, producing polysaccharide hydrolyzing enzymes, remains a major bottle neck for efficient utilization of bioactive algal oligosaccharides. This review summarizes the recent developments in the identification and characterization of microbial enzymes for the production of bioactive algal oligosaccharides. This can improve our understanding of bioactive algal oligosaccharides and pave way for efficient utilization of macroalgae to prevent various chronic diseases.

全球对藻类食物的需求正在增加，因为它们的营养和健康被逐渐消耗。大型藻类是经过验证的代谢物、蛋白质、色素、生物活性化合物和藻类多糖的来源。独特的多糖，如琼脂、角叉菜胶、卟啉、藻酸盐、岩藻多糖、海带多糖和乌尔万以其广泛的生物活性而闻名，并广泛用于从组织工程到药物输送的应用。然而，由于其高分子量、低相容性和亲水胶体性质，几乎没有限制。因此，酶法生产的藻寡糖由于其绿色合成、溶解性和较小的分子尺寸而引起了极大的关注。据报道，它们具有生物活性，包括抗氧化、降血糖、免疫刺激、抗炎、和益生元活性，可用于医疗保健和保健品行业，用于制造功能性食品和膳食补充剂。然而，潜在微生物的鉴定，产生多糖水解酶，仍然是有效利用生物活性藻类寡糖的主要瓶颈。本综述总结了用于生产生物活性藻寡糖的微生物酶的鉴定和表征的最新进展。这可以提高我们对生物活性藻类低聚糖的理解，为有效利用大型藻类预防各种慢性疾病铺平道路。产生多糖水解酶，仍然是有效利用生物活性藻类寡糖的主要瓶颈。本综述总结了用于生产生物活性藻寡糖的微生物酶的鉴定和表征的最新进展。这可以提高我们对生物活性藻类低聚糖的理解，为有效利用大型藻类预防各种慢性疾病铺平道路。产生多糖水解酶，仍然是有效利用生物活性藻类寡糖的主要瓶颈。本综述总结了用于生产生物活性藻寡糖的微生物酶的鉴定和表征的最新进展。这可以提高我们对生物活性藻类低聚糖的理解，为有效利用大型藻类预防各种慢性疾病铺平道路。