Background: Conventional analytical methods for phosphorus and sulfur determination in several matrices present normally analytical challenges regarding inaccuracy, detectability and waste generation.

Objective: The main objective is proposing a green and feasible analytical method for phosphorus and sulfur determination in animal feed.

Methods: Synergic effect between microwave and ultraviolet radiations during sample preparation was evaluated for the first time for the animal feed digestion associated with further phosphorus and sulfur determination by ion chromatography with conductivity detection. Dissolved carbon and residual acidity in the final digests were used for the proposed method assessment. Phosphorus and sulfur values were compared with those obtained using conventional microwaveassisted wet digestion in closed vessels associated with inductively coupled plasma optical emission spectrometry and with those obtained using an official method (Association of Official Analytical Chemists International). Recovery tests and certified reference material analyses were performed. Animal feeds were analyzed using the proposed method.

Results: Sample masses of 500 mg were efficiently digested using only 2 mol L^-1 HNO3. The results obtained by the proposed method were not differing significantly (p > 0.05) from those obtained by conventional and by official methods. Suitable recoveries (from 94 to 99%), agreement with certified values (101 and 104%), and suitable relative standard deviations (< 8%) were achieved. Phosphorus and sulfur content in commercial products varied in a wide range (P: 5,873 to 28,387 mg kg^-1 and S: 2,165 to 4,501 mg kg^-1).

Conclusion: The proposed method is a green, safe, accurate, precise and sensitive alternative for animal feed quality control.

背景：用于在多种基质中测定磷和硫的常规分析方法通常会带来有关准确性，可检测性和废物产生的分析难题。

目的：主要目的是为动物饲料中的磷和硫的测定提出一种绿色可行的分析方法。

方法：首次评估了样品制备过程中微波和紫外线之间的协同作用，用于动物饲料的消化，并通过电导检测离子色谱法进一步测定磷和硫。最终消化液中的溶解碳和残留酸度用于拟议方法评估。将磷和硫的值与使用常规微波辅助湿法在密闭容器中与电感耦合等离子体光发射光谱法相关的结果和使用官方方法（国际分析化学家协会）获得的结果进行了比较。进行了恢复测试和认证的参考物质分析。使用提出的方法分析动物饲料。

结果：仅使用2 mol L ^-1 HNO3可有效消化500 mg样品质量。通过提议的方法获得的结果与通过常规方法和通过官方方法获得的结果没有显着差异（p> 0.05）。获得了合适的回收率（从94％到99％），与认证值一致（101和104％）以及合适的相对标准偏差（<8％）。商业产品中的磷和硫含量变化很大（P：5,873至28,387 mg kg ^-1和S：2,165至4,501 mg kg ^-1）。

结论：该方法是一种绿色，安全，准确，精确和灵敏的动物饲料质量控制方法。