当前位置: X-MOL 学术Cereal Res. Commun. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Molecular mapping of a novel QTL for resistance to cereal cyst nematode in F4 wheat population
Cereal Research Communications ( IF 1.6 ) Pub Date : 2021-03-24 , DOI: 10.1007/s42976-021-00159-9
Talal K. Al-Ateeq , Abdullah A. Al-Doss , Ahmad S. Al-Hazmi , Abdelhalim I Ghazy , Ahmed M. Dawabah , Mohamed I. Motawei

A wheat line selected from an off-type plant (Lnm136) was found to be resistant to Heterodera avenae (cereal cyst nematode, CCN). A F4 population developed by crossing this line with a CCN susceptible genotype, Ksu118, was used to study the map location of the CCN resistance. The results showed that the most significant QTLs for the cereal cyst nematode (CCN) score were identified on five chromosomes. One of the QTLs was located on chromosome 1B and linked closely to Xwmc85 based on composite interval mapping. A novel locus accounting for up to 70% of the resistance to CCN was mapped to chromosome 1B of Lnm136, by association with the microsatellite marker Xwmc85-1B. The selected CCN resistant F4 lines showed introgression for molecular markers identified to be linked with the CCN resistance locus Xwmc85-1B. This QTL on chromosome 1BS in the interval of Xwmc85–Xgpw4331 appears to be a major novel resistance locus. Multiple regression analysis showed the importance of the 1B region and marker-assisted selection for the favorable allele at this region should improve the resistance against CCN. The introgression lines can be used for marker-assisted transfer of Xwmc85-1B to elite wheat cultivars.



中文翻译:

F4小麦群体中新型抗谷物囊肿线虫QTL的分子图谱

发现选自异型植物(Lnm136)的小麦品系对燕麦杂种优势(谷物囊肿线虫,CCN)具有抗性。通过将这条线与CCN易感基因型Ksu118杂交而开发的AF 4种群用于研究CCN抗性的定位图。结果表明,谷物囊肿线虫(CCN)得分的最显着QTL在5条染色体上得到了识别。QTL之一位于染色体1B上,并根据复合间隔图与Xwmc85紧密相连。通过与微卫星标记Xwmc85 -1B结合,将一个占最高70%对CCN抗性的新基因座定位到Lnm136的1B号染色体。所选的抗CCN F 4品系显示鉴定为与CCN抗性基因座Xwmc85 -1B相关的分子标记的渗入。在Xwmc85–Xgpw4331区间的1BS染色体上的这个QTL似乎是一个主要的新抗性基因座。多元回归分析表明1B区域的重要性,在该区域有利于等位基因的标记辅助选择应可提高对CCN的抵抗力。渐渗线可用于Xwmc85 -1B的标记辅助转移至优良小麦品种。

更新日期:2021-03-25
down
wechat
bug