Boolean logic gate based on DNA strand displacement for biosensing: current and emerging strategies,Nanoscale Horizons

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Boolean logic gate based on DNA strand displacement for biosensing: current and emerging strategies
Nanoscale Horizons ( IF 8.0 ) Pub Date : 2021-3-24 , DOI: 10.1039/d0nh00587h
Shuang Zhao ₁ , Lianyu Yu , Sha Yang , Xiaoqi Tang , Kai Chang , Ming Chen

Affiliation

DNA computers are considered one of the most prominent next-generation molecular computers that perform Boolean logic using DNA elements. DNA-based Boolean logic gates, especially DNA strand displacement-based logic gates (SDLGs), have shown tremendous potential in biosensing since they can perform the logic analysis of multi-targets simultaneously. Moreover, SDLG biosensors generate a unique output in the form of YES/NO, which is contrary to the quantitative measurement used in common biosensors. In this review, the recent achievements of SDLG biosensing strategies are summarized. Initially, the development and mechanisms of Boolean logic gates, strand-displacement reaction, and SDLGs are introduced. Afterwards, the diversified input and output of SDLG biosensors are elaborated. Then, the state-of-the-art SDLG biosensors are reviewed in the classification of different signal-amplification methods, such as rolling circle amplification, catalytic hairpin assembly, strand-displacement amplification, DNA molecular machines, and DNAzymes. Most importantly, limitations and future trends are discussed. The technology reviewed here is a promising tool for multi-input analysis and lays a foundation for intelligent diagnostics.

中文翻译：

基于 DNA 链置换的布尔逻辑门用于生物传感：当前和新兴策略

DNA 计算机被认为是最杰出的下一代分子计算机之一，它使用 DNA 元素执行布尔逻辑。基于 DNA 的布尔逻辑门，尤其是基于 DNA 链置换的逻辑门 (SDLG)，由于它们可以同时执行多目标的逻辑分析，因此在生物传感方面显示出巨大的潜力。此外，山东临工生物传感器以 YES/NO 的形式产生独特的输出，这与普通生物传感器中使用的定量测量相反。在这篇综述中，总结了山东临工生物传感策略的最新成果。首先介绍了布尔逻辑门、链置换反应和 SDLG 的发展和机制。随后，详细阐述了临工生物传感器的多元化输入输出。然后，最先进的 SDLG 生物传感器在不同信号放大方法的分类中进行了审查，例如滚环放大、催化发夹组装、链置换放大、DNA 分子机器和 DNAzyme。最重要的是，讨论了局限性和未来趋势。这里审查的技术是多输入分析的有前途的工具，并为智能诊断奠定了基础。

更新日期：2021-03-25

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