Patients with chronic pain develop peripheral neuropathy and experience sleep disturbance. Yokukansan is used to treat insomnia and control neuropathic pain. We studied if Yokukansan affects neuropathic pain and sleep disturbance using a rat model of chronic constriction injury (CCI). Male Wistar rats (4-week age) were divided into the following groups (n = 7, per group): CCI rats fed normal chow (CCI-0); CCI rats fed powdered chow mixed with 1% Yokukansan (CCI-1); CCI rats fed powdered chow mixed with 3% Yokukansan (CCI-3); and sham-operated control rats fed normal chow (SHAM). We examined sleep duration and quality using electroencephalograms and assessed pain using the von Frey and Hargreaves tests. Results were analyzed by one-way analysis of variance and Bonferroni post hoc tests. The CCI-0 group exhibited an increased wake period, decreased non-rapid eye movement (REM) sleep time, and no change in REM sleep time in comparison to the SHAM group. The CCI-1 group exhibited a decreased wake period, increased non-REM sleep time, and no change in REM sleep time compared to the CCI-0 group. The CCI-3 group exhibited increased non-REM sleep time but no changes in wake and REM sleep times compared to the CCI-1 group. The von Frey and Hargreaves test findings revealed an increase in the pain threshold in the CCI-1 group compared to the CCI-0 group. There was no difference in pain threshold between the CCI-1 and CCI-3 groups. In our rat model of CCI, sleep disturbance was reflected. Yokukansan inhibited CCI-induced sleep disturbance.

患有慢性疼痛的患者会发展为周围神经病并经历睡眠障碍。Yokukansan用于治疗失眠和控制神经性疼痛。我们使用慢性收缩损伤（CCI）大鼠模型研究了Yokukansan是否会影响神经性疼痛和睡眠障碍。将雄性Wistar大鼠（4周龄）分为以下各组（n = 7，每组）：CCI大鼠饲喂正常食物（CCI-0）；CCI大鼠喂食与1％Yokukansan（CCI-1）混合的粉状食物；CCI大鼠饲喂与3％Yokukansan（CCI-3）混合的粉状食物；和假手术的对照组大鼠喂食正常食物（SHAM）。我们使用脑电图检查了睡眠时间和质量，并使用von Frey和Hargreaves测试评估了疼痛。通过单因素方差分析和Bonferroni事后检验对结果进行分析。与SHAM组相比，CCI-0组表现出增加的唤醒时间，减少的非快速眼动（REM）睡眠时间，并且REM睡眠时间没有变化。与CCI-0组相比，CCI-1组的唤醒时间减少，非REM睡眠时间增加，并且REM睡眠时间没有变化。与CCI-1组相比，CCI-3组的非REM睡眠时间增加，但苏醒和REM睡眠时间无变化。von Frey和Hargreaves的测试发现表明，与CCI-0组相比，CCI-1组的疼痛阈值有所增加。CCI-1和CCI-3组之间的疼痛阈值无差异。在我们的大鼠CCI模型中，反映了睡眠障碍。Yokukansan抑制了CCI引起的睡眠障碍。