The purpose of this study was to assess the effects of dietary humate substances (HS) and CloSTAT (Bacillus subtilis PB6) on the thyroid activity and histology, iron profile, blood haematology and performance of growing Japanese quail. A total of 216 unsexed 7-day-old quail chicks were randomly assigned to six groups. The first group was fed a basal diet (BD) without any additives (control); the 2nd group received BD plus 0.05% CloSTAT, the 3rd and 4th groups were given BD plus 0.4% and 0.8% HS, respectively; and the 5th and 6th groups were administered BD plus CloSTAT + 0.4% HS and BD plus CloSTAT + 0.8% HS, respectively. The results showed that the growth performance was improved with the addition of CloSTAT alone or in combination with 0.4% HS compared with the control. Haematological parameters, iron level and transferrin saturation % were significantly (p < 0.001) increased by feeding HS compared with the control group. Serum thyroxin and triiodothyronine levels were significantly (p = 0.001) increased by adding CloSTAT relative to the control. Supplementation of 0.8% HS caused deterioration in histomorphometry parameters of the thyroid gland, but these parameters were improved in response to CloSTAT compared with the control. In conclusion, dietary B. subtilis PB6 as CloSTAT or CloSTAT + 0.4% HS supplementation may be efficacious in enhancing the growth performance and boosting the thyroid activity of growing Japanese quail. Moreover, the addition of 0.4% or 0.8% HS to quail diets boosted their iron profile and haematological parameters.

中文翻译：

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腐植酸盐物质和枯草芽孢杆菌 PB6 对鹌鹑甲状腺活性和组织形态学、铁含量和血液学的影响

本研究的目的是评估膳食腐植酸盐物质 (HS) 和 CloSTAT (枯草芽孢杆菌PB6) 对生长日本鹌鹑的甲状腺活动和组织学、铁谱、血液学和性能的影响。总共有 216 只无性别的 7 日龄鹌鹑小鸡被随机分配到六组。第一组喂食不含任何添加剂的基础日粮（BD）（对照）；第2组给予BD加0.05% CloSTAT，第3组和第4组分别给予BD加0.4%和0.8% HS；第 5 组和第 6 组分别给予 BD 加 CloSTAT + 0.4% HS 和 BD 加 CloSTAT + 0.8% HS。结果表明，与对照相比，单独添加 CloSTAT 或与 0.4% HS 联合添加可提高生长性能。血液学参数、铁水平和转铁蛋白饱和度%显着（p < 0.001) 与对照组相比，喂食 HS 增加。 相对于对照，添加 CloSTAT 可显着增加血清甲状腺素和三碘甲状腺原氨酸水平 ( p = 0.001)。补充 0.8% HS 会导致甲状腺组织形态学参数的恶化，但与对照相比，这些参数在使用 CloSTAT 时得到了改善。总之，作为 CloSTAT 或 CloSTAT + 0.4% HS 补充剂的日粮枯草芽孢杆菌PB6 可能有效提高日本鹌鹑的生长性能和甲状腺活性。此外，在鹌鹑日粮中添加 0.4% 或 0.8% HS 可提高其铁含量和血液学参数。