ABSTRACT

Nerol (cis-3,7-dimethyl-2,6-octadien-1-ol) is a monoterpene widely used in cosmetic products, household detergents and cleaners, as well as a flavoring in several food products. Despite the high level of human exposure to nerol, an absence of studies regarding potential genetic toxicity in human cells exists. The aim of this investigation was to examine the cytotoxic and genotoxic potential of this monoterpene on human peripheral blood mononuclear cells as well as hepatic metabolizing HepG2/C3A human cell line. Cytotoxicity was assessed using trypan blue staining and MTT assay while genotoxicity was determined utilizing the comet and micronucleus test. Cytotoxicity tests showed cell viability greater than 70% for concentrations between 2.5 and 500 µg/ml. Both cell types exhibited significant DNA damage and chromosomal mutations after medium and high concentration incubation with nerol indicating that the safety of use of this monoterpene in various formulations to which humans are exposed needs to be monitored and requires more comprehensive investigations.

摘要

橙花（顺式-3,7-二甲基-2,6-octadien-1-ol) 是一种单萜，广泛用于化妆品、家用洗涤剂和清洁剂，以及多种食品中的调味剂。尽管人类对橙花醇的暴露水平很高，但缺乏关于人类细胞潜在遗传毒性的研究。本研究的目的是检查这种单萜对人外周血单核细胞以及肝脏代谢 HepG2/C3A 人细胞系的细胞毒性和基因毒性潜力。使用台盼蓝染色和 MTT 测定评估细胞毒性，而使用彗星和微核试验确定遗传毒性。细胞毒性试验表明，浓度介于 2.5 和 500 µg/ml 之间时，细胞活力大于 70%。