Both fast seed germination and seedling vigour are important for proper crop establishment and higher yields. These traits seem to be controlled by several QTL’s and are highly influenced by environmental conditions. In this work, we aimed to evaluate seed germination performance and vigour traits of several Solanum pennellii introgression lines (IL’s) to select ideotypes for germination and vigour; and then investigate the selected IL’s to uncover candidate genes for these traits. We performed a germination test followed by the analysis of seedling growth in a collection of 46 different homozygous tomato IL’s and their genitors' accession LA716 and cv M82, grown in the field in two consecutive growing seasons. From these tests, several traits were calculated. Then, we used a multi-trait index based on factor analysis and genotype-ideotype distance (FAI-BLUP index) to properly rank the genotypes according to their seed germination performance and seedling vigour. Following the ranking, introgressed segments of interest were identified for further analysis of candidate gene identification using the map of the tomato IL population as provided by the SOL Genomics Network. The IL 6−1, IL 4−1−1, IL 4−4, IL 11−4−1, IL 1−1−2, and IL 10−1 were selected through the FAI-BLUP index as the closest to the ideotype for seed germination performance and vigour. The genes At1g75350, At2g26350, At2g28490, At3g10920, At5g13200, At5g41480, and At4g21800 were identified as candidate genes for seed germination. The genes At5g53000, At1g46480, At1g30755, At5g47390, At2g29630, and At4g03400 were identified as candidate genes for seed vigour. Fine mapping, validation, and further investigation of these genes will provide valuable insights into the genetics of seed quality.

快速的种子发芽和幼苗活力对于适当的作物生长和更高的产量都至关重要。这些特征似乎受多个QTL的控制，并且受环境条件的影响很大。在这项工作中，我们旨在评估几种茄属茄科植物的种子发芽性能和活力性状。渗入系（IL）选择用于发芽和活力的表型；然后研究选定的IL，以发现这些性状的候选基因。我们进行了发芽测试，然后分析了在连续两个生长季节中在田间生长的46种不同的纯合番茄IL及其祖先的登录号LA716和cv M82的集合中的幼苗生长。从这些测试中，计算出几个特征。然后，基于因子分析和基因型-意识型距离（FAI-BLUP指数），使用多性状指标根据种子的发芽性能和幼苗活力适当地对基因型进行排名。排名之后 使用SOL Genomics Network提供的番茄IL群体图谱，鉴定了感兴趣的基因渗入部分，以进一步分析候选基因。通过FAI-BLUP索引选择IL 6-1，IL 4-1，IL 4-4，IL 11-4--1，IL 1-1和IL 10-1作为最接近的指标。种子发芽表现和活力的独特型。基因At1g75350，At2g26350，At2g28490，At3g10920，At5g13200，At5g41480和At4g21800被确定为种子发芽的候选基因。已将基因At5g53000，At1g46480，At1g30755，At5g47390，At2g29630和At4g03400确定为种子活力的候选基因。这些基因的精细定位，验证和进一步研究将为种子品质的遗传学提供有价值的见解。