Heparan sulfate proteoglycans consist of a small family of proteins decorated with one or more covalently attached heparan sulfate glycosaminoglycan chains. These chains have intricate structural patterns based on the position of sulfate groups and uronic acid epimers, which dictate their ability to engage a large repertoire of heparan sulfate–binding proteins, including extracellular matrix proteins, growth factors and morphogens, cytokines and chemokines, apolipoproteins and lipases, adhesion and growth factor receptors, and components of the complement and coagulation system. This review highlights recent progress in the characterization of the so-called “heparan sulfate interactome,” with a major focus on systems-wide strategies as a tool for discovery and characterization of this subproteome. In addition, we compiled all heparan sulfate–binding proteins reported in the literature to date and grouped them into a few major functional classes by applying a networking approach.

硫酸乙酰肝素蛋白聚糖由一小部分蛋白质组成，这些蛋白质装饰有一个或多个共价连接的硫酸乙酰肝素糖胺聚糖链。这些链基于硫酸盐基团和糖醛酸差向异构体的位置具有复杂的结构模式，这决定了它们与大量硫酸乙酰肝素结合蛋白结合的能力，这些蛋白包括细胞外基质蛋白，生长因子和形态发生子，细胞因子和趋化因子，载脂蛋白和脂肪酶，粘附和生长因子受体，以及补体和凝血系统的成分。这篇综述着重介绍了在所谓的“硫酸乙酰肝素相互作用组”的表征方面的最新进展，主要侧重于系统范围内的策略，作为发现和表征该蛋白质组的工具。此外，