We developed a novel real-time PCR assay for rapid sexing in all three elephant species, which amplifies small fragments of the orthologous sexual chromosome zinc finger protein genes ZFX/ZFY (65 bp). This assay is a simple, inexpensive and reliable tool that is suitable for non-invasive DNA samples and can be incorporated into larger SNP panels for individual identification and population genetic studies.

中文翻译：

---

大象物种的简单性别鉴定及其在粪便 DNA 中的应用

我们开发了一种新的实时 PCR 检测方法，用于在所有三种大象物种中进行快速性别鉴定，它可以放大直向同源性染色体锌指蛋白基因 ZFX/ZFY（65 bp）的小片段。该检测是一种简单、廉价且可靠的工具，适用于非侵入性 DNA 样本，并可纳入更大的 SNP 面板以进行个体识别和群体遗传研究。