The first barrier, when penetrating an insect host, encountered by microorganisms like fungi is insects cuticle; fungi produce a wide variety of extracellular enzymes involved in the degradation of protein, chitin, and lipids. The main objectives were to assay the enzymatic activity of Cordyceps locustiphila anamorph, in solid and liquid medium at different temperatures and also to evaluate the pathogenicity against the South American locust, Schistocerca cancellata. Conidia of C. locustiphila anamorph were adjusted to 1 × 10⁴, 1 × 10⁶ and 1 × 10⁸ conidia/ml and mortality was recorded for S. cancellata nymphs. The enzymatic activities were determined through a plate test and liquid medium. When assessing the pathogenicity, the fungus caused the highest mortality (84.5 ± 3.5%) at a dose of 1 × 10⁸ conidia/ml. The proteolytic activity showed the highest values (1.56 ± 0.21) (U) at 26 °C. The highest lipolytic activity (1.13 ± 0.36) (U) was observed at 26 °C, while the highest chitinolytic activity was of 0.85 ± 0.1 (U) at 4 °C. Significant differences were observed for enzymatic production on liquid medium, the highest values were recorded for chitinolytic activity (1.63 ± 0.04) (U) and the lowest for caseinolytic activity (0.04 ± 0.001) (U). The results obtained on mortality as well as the quality and variety of enzymes produced by C. locustiphila suggest that this fungus has features that make it a good candidate to be used as a biological agent for the control of the South American locust.

真菌等微生物在穿透昆虫宿主时遇到的第一个障碍是表皮昆虫。真菌产生与蛋白质，甲壳质和脂质降解有关的多种细胞外酶。其主要目的是测定酶活性虫草locustiphila无性型，固体和液体培养基中，在不同的温度，并以评估对南美蝗虫，致病性蝗cancellata。弯孢菌无性分生孢子的分生孢子调整为1×10 ^4，1 ×10 ⁶和1×10 ⁸分生孢子/ ml，并记录了S. cancellata的死亡率若虫。通过平板试验和液体培养基确定酶活性。在评估致病性时，真菌以1×10 ⁸分生孢子/ ml的剂量引起最高死亡率（84.5±3.5％）。蛋白水解活性在26°C时显示最高值（1.56±0.21）（U）。在26°C时观察到最高的脂解活性（1.13±0.36）（U），而在4°C时最高的壳多糖分解活性为0.85±0.1（U）。观察到液体培养基上酶促生产的显着差异，记录的几丁质分解酶活性最高（1.63±0.04）（U），最低的酪蛋白分解活性（0.04±0.001）（U）。在死亡率，以及由C. locustiphila产生的酶的质量和种类方面获得的结果 这表明这种真菌具有使其成为控制南美蝗虫的生物制剂的良好候选物的特征。