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The use of roller swabs for Salmonella detection in poultry litter
Journal of Applied Poultry Research ( IF 1.9 ) Pub Date : 2021-03-23 , DOI: 10.1016/j.japr.2021.100163
A.A. Talorico , M.A. Bailey , L.R. Munoz , K.S. Chasteen , A. Pal , J.T. Krehling , D.V. Bourassa , R.J. Buhr , K.S. Macklin

Litter sampling is utilized as a noninvasive and practical method to determine broiler flock Salmonella status. The common methods include boot cover/sock (BC), drag swab (DS), or litter grab sampling (LG). Roller swabs are a new research method that can be used to sample litter without entering research pens. This study aimed to assess the use of roller swabs (RS) for Salmonella (S) detection in comparison to BC, DS, and LG. For Experiment 1, litter was sampled for two weeks following a broiler flock that was challenged at 6 days of age with 1 × 107cfu of a nalidixic acid resistant strain of Salmonella Enteritidis to establish a high litter prevalence of S. In Experiment 2, sampling occurred after a subsequent flock was raised on the same litter. In Experiment 1, S was detected by RS less frequently (81%) than DS (95%), BC (97%), and LG (98%; P < 0.0001). In Experiment 2, S detection using RS (23%) was similar to LG (17%), higher than DS (6%, P = 0.022), and lower than BC (55%, P < 0.0001). Although RS were a less sensitive sampling method when the litter prevalence of S was high, RS were equivalent to or better than LG and DS methods when S prevalence was low. The use of roller swabs allows for sampling of litter without entering each pen and has the potential to be utilized for Salmonella detection in research pen trials.



中文翻译:

轮状拭子在家禽垫料中沙门氏菌检测中的应用

凋落物采样​​被用作确定肉鸡沙门氏菌状况的一种非侵入性实用方法。常用的方法包括靴套/袜子(BC),拖把(DS)或垃圾抓取采样(LG)。滚拭子是一种新的研究方法,可用于对垃圾进行采样,而无需进入研究笔。这项研究的目的是评估使用辊拭子(的RS对)沙门氏菌小号相比BC,DS,和LG)检测。对于实验1,在1天10天用1×10 7 cfu耐萘啶酸的沙门氏菌菌株攻击的肉鸡群中,对仔猪采样两周。肠炎沙门氏菌患病率较高。在实验2中,在同一窝产仔数增加后进行采样。在实验1中,通过RS检出S的频率低于DS(95%),BC(97%)和LG(98%;P <0.0001)的频率(81%)。在实验2中,使用RS的S检测(23%)与LG(17%)相似,高于DS(6%,P  = 0.022),低于BC(55%,P <0.0001)。尽管当S的患病率较高时,RS是不太敏感的采样方法,但当S的患病率较低时,RS等于或优于LG和DS方法。滚轮拭子的使用可在不进入每支笔的情况下对垃圾进行采样,并有可能被用于研究笔试验中的沙门氏菌检测。

更新日期:2021-05-13
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