In sweet cherry, as in most non-climacteric species, abscisic acid (ABA) plays a major role in the control of fruit ripening and color development. Although the ABA treatment of sweet cherry fruits has been reported to upregulate anthocyanin pathway-related genes or ABA pathway-related genes, the temporality of molecular and physiological events occurring during color development and the ABA control of these events during the color initiation are lacking in this species. In this work, we analyzed variations in the Index of Absorbance Difference (IAD), a maturity index, and total anthocyanins along with changes in transcript abundance of ABA and anthocyanin pathway-related genes, from light green to red fruit stages. PavNCED1 and ABA signaling pathway-related genes upregulated when fruits transitioned from light green to pink stage, whereas anthocyanin pathway-related transcripts increased from pink to the red stage, together with increases in the anthocyanin content and IAD, suggesting sequentiality in molecular and physiological events during color development. Additionally, ABA applied at color initiation in planta advanced IAD, increased anthocyanin content, and yielded darker fruits at harvest. These changes were accompanied by changes in the transcript accumulation of ABA and anthocyanin pathway-related genes. This in planta treatment of sweet cherry fruits with ABA confirms that ABA is a central player in the control of color initiation in sweet cherries, associated with the transcript accumulation of genes involved in ABA homeostasis and signaling, which is followed by the up-regulation of anthocyanin pathway-related genes and color development.

与大多数非更年期品种一样，在甜樱桃中，脱落酸（ABA）在控制果实成熟和发色方面起着重要作用。虽然有报道称用ABA处理甜樱桃果实会上调花色苷途径相关基因或ABA途径相关基因，但在色泽发育过程中发生的分子和生理事件的时间性以及在颜色起始过程中对这些事件的ABA控制均缺乏。这个物种。在这项工作中，我们分析了吸光度差异指数（IAD），成熟度指数和总花色苷的变化，以及从浅绿色到红色果实阶段的ABA和花色苷途径相关基因的转录丰度变化。PavNCED1水果从浅绿色过渡到粉红色阶段时，ABA和ABA信号通路相关基因的表达上调，而花色苷途径相关的转录物从粉红色到红色阶段增加，同时花色苷含量和IAD升高，表明在此期间分子和生理事件的顺序性显色。此外，ABA在植物的IAD上色开始时就使用了先进的IAD，增加了花色苷含量，并在收获时产生了较深色的果实。这些变化伴随着ABA和花色苷途径相关基因的转录物积累的变化。这个在植物中 用ABA处理甜樱桃果实证实了ABA是控制甜樱桃中颜色起始的关键因素，这与参与ABA稳态和信号传导的基因的转录物积累有关，随后是花色苷途径的上调-相关基因和颜色的发展。