Streptomyces sp. 2M21 was evaluated for keratinase production in bioreactors using chicken feathers. Firstly, optimization of bioengineering parameters (agitation and aeration rates) using Response Surface Methodology was carried out in 2 L bioreactors. Optimized conditions identified by the modified quadratic model were verified as 150 rpm and 1 vvm experimentally corresponding to 351 U/ml of keratinase activity. Moreover, scaling up sequentially to 20 L bioreactors was implemented using constant impeller tip speed and constant mass transfer coefficient as key scale-up parameters. The keratinase activity in 5, 10 and 20 L bioreactors showed similar results with the one of shake flasks (412 U/ml) and 2 L bioreactors (351 U/ml)with respect to the keratinase activity values of 336, 385 and 344 U/ml, respectively. The results suggest keratinase production by evaluating chicken feathers in commercial level. Furthermore, this study has potential to contribute industrial scale production of keratinase by Streptomyces sp. 2M21 using the proposed bioreactor conditions.

链霉菌sp. 在使用鸡毛的生物反应器中评估了 2M21 的角蛋白酶生产。首先，在 2 L 生物反应器中使用响应面方法优化生物工程参数（搅拌和曝气速率）。由改进的二次模型确定的优化条件被证实为 150 rpm 和 1 vvm，实验上对应于 351 U/ml 的角蛋白酶活性。此外，使用恒定的叶轮尖端速度和恒定的传质系数作为关键放大参数，按顺序放大到 20 L 生物反应器。在 336、385 和 344 U 的角蛋白酶活性值方面，5、10 和 20 L 生物反应器中的角蛋白酶活性与摇瓶 (412 U/ml) 和 2 L 生物反应器 (351 U/ml) 之一显示出相似的结果/ml，分别。结果表明通过评估商业水平的鸡毛来生产角蛋白酶。此外，这项研究有可能通过以下方式促进角蛋白酶的工业规模生产链霉菌属 2M21 使用建议的生物反应器条件。