Global demands for an in vitro culture of cannabis have never been more sought after as countries shift their paradigm towards legalization. Cannabis conventional (photomixotrophic) micropropagation has not been suitable enough for large-scale propagation due to a high degree of plant hyperhydricity, low growth rate, poor rooting, and acclimation efficiency. In the present study, cannabis photoautotrophic micropropagation method is introduced with the purpose of overcoming the difficulties that conventional micropropagation entails when conducted at a large scale. The roles of rockwool medium pH and moisture content, cutting length, basal wounding methods, light intensity, and culture vessel gas exchange capacity were assessed with the intention of increasing productivity of micropropagation method. The results showed 300 mL per vessel of fertilizer solution containing 5-mM MES buffer stabilized medium pH and increased rooting success. Both 5- and 7-cm cutting lengths significantly increased the percent of rooted plants compared to 3-cm cutting length. However, the basal wounding methods did not significantly improve or impede the rooting success. The highest rooting success was also obtained with 150 μmol m⁻² s⁻¹ of photosynthetic photon flux density compared to 50 and 100 μmol m⁻² s⁻¹. Increasing gas exchange rates either using more permeable vessels or aeration practices significantly improved the rooting success. Overall, more than 90% of cannabis plantlets grown in photoautotrophic micropropagation are rooted in 2 wk of culture followed by 4 d ex vitro acclimation period, which was remarkably shorter than any other available method in cannabis micropropagation. This study not only optimizes a method for cannabis photoautotrophic micropropagation using passive ventilation for the first time but also scales up in vitro clonal propagation for in vitro commercial production.

全球对体外的需求随着各国将范式转向合法化，大麻文化从未受到过追捧。大麻的常规（光合营养）微繁殖由于高度的植物高水度，低生长速率，较差的生根和驯化效率而不适用于大规模繁殖。在本研究中，引入大麻光合自养微繁方法的目的是克服常规的微繁大规模进行时所带来的困难。为了提高微繁殖方法的生产率，评估了岩棉培养基的pH和水分含量，切割长度，基础伤口处理方法，光强度和培养容器气体交换能力的作用。结果表明，每容器含5-mM MES缓冲液的肥料溶液300 mL可稳定培养基pH值并提高生根成功率。与3厘米的切割长度相比，5厘米和7厘米的切割长度都显着增加了生根植物的百分比。然而，基础伤的方法并没有显着改善或阻碍生根成功。150μmolm也获得了最高的生根成功率^-2 š ^-1相比50和100μmol米光合光子通量密度的^-2小号^-1。使用更多的可渗透容器或通气方法来提高气体交换速率，可显着提高生根成功率。总体而言，在光合自养微繁殖中生长的大麻苗有90％以上植根于2 wk的培养物中，随后是4 d的体外适应期，这比任何其他在大麻微繁殖中可用的方法都要短。这项研究不仅优化使用被动通风首次大麻光自养微量繁殖的方法，还可扩展至体外克隆繁殖用于体外 商业生产。