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A practical, low-cost, short-term storage method for genomic DNA.
Biotechniques ( IF 2.2 ) Pub Date : 2021-03-22 , DOI: 10.2144/btn-2020-0158
Demir Özdemir 1
Affiliation  

The aim of this study was to assess the DNA preservation capability of cellulose paper towel and blotting paper as low-cost alternatives to commercial DNA preservation products. Chicken blood was applied as DNA source to each paper towel, blotting paper, FTA® cards and DNA/RNA Shield™. All samples were stored at room temperature for 130 days. DNA extraction from dried blood spots was performed after various time periods using Tris-EDTA and NaOH protocols. PCR activity and the mean amount of DNA isolated from paper towels were reliable. The results of this study demonstrated that cellulose-based blotting paper and especially paper towel had considerable DNA binding and preservation capacity for at least 130 days at room temperature without DNA degradation.

中文翻译:

一种实用、低成本、短期的基因组 DNA 存储方法。

本研究的目的是评估纤维素纸巾和吸墨纸作为商业 DNA 保存产品的低成本替代品的 DNA 保存能力。将鸡血作为 DNA 来源应用于每张纸巾、吸墨纸、FTA ®卡片和 DNA/RNA Shield™。所有样品均在室温下储存 130 天。在不同时间段后使用 Tris-EDTA 和 NaOH 方案从干血斑中提取 DNA。PCR 活性和从纸巾中分离的平均 DNA 量是可靠的。这项研究的结果表明,基于纤维素的吸墨纸,尤其是纸巾,在室温下至少可以保持 130 天的 DNA 结合和保存能力,而且 DNA 不会降解。
更新日期:2021-03-29
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