The growth and survival of pearl oyster postlarvae (Pinctada martensii) were studied under laboratory conditions. A face-centered central composite experimental design was used with 3 factors, temperature (18―34 °C), algal ration (5―100 cells μL^―1) and stocking density (0.2―3.0 in.. mL^―1). Pearl oyster postlarvae were fed with a 1: 1 mixture of Isochrysis zhanjiangensis and Pavlova viridis during the experiment. Temperature, algal ration and stocking density exerted significant effects on postlarvae growth and survival. Temperature was the most dominant factor, followed by algal ration. Significant interactions among the 3 factors were inhibitory (negative) to growth and survival. Within the ranges of 3 factors tested, growth and survival increased with 3 factors up to a pinnacle, beyond which they began to decrease. The conditions required for the optimal growth could also ensure the optimal survival. Through simultaneously maximizing the reliable models of postlarvae growth and survival, the optimal trifactor culture conditions of 27.86 °C/50.85 cells μL^―1/1.60 in.. mL^―1 were obtained. These optimal culture conditions resulted in the greatest specific growth of 8.95 % d^―1 (95 % confidence interval, 8.73―9.18 % d^―1) and survival of 21.5 % (95 % confidence interval, 21.06―21.94 %), with desirability as high as 99.6 %. Due to practical feasibility, the optimal trifactor culture conditions were recommended to apply to practice to maximize growth and survival of the pearl oyster P. martensii postlarvae martensii. This study provides valuable new insights into refining the production efficiency of the pearl oyster postlarvae.

在实验室条件下研究了牡蛎后期幼体（Pinctada martensii）的生长和存活。使用以面部为中心的中央复合实验设计，该设计具有3个因素，温度（18-34°C），藻类定量（5-100个细胞μL ^-1）和放养密度（0.2-3.0 in .. mL ^-1）。给珍珠牡蛎后幼体喂食湛江等量线虫和绿色巴甫洛娃的1：1混合物在实验过程中。温度，藻类比例和放养密度对幼虫的生长和存活产生重要影响。温度是最主要的因素，其次是藻类定量。这三个因素之间的显着相互作用是抑制（阴性）生长和存活。在测试的3个因素的范围内，生长和存活率随着3个因素的增加而增加，直至达到顶峰，然后开始下降。最佳生长所需的条件也可以确保最佳生存。通过同时最大化幼虫后生长和存活的可靠模型，最佳三因子培养条件为27.86°C / 50.85细胞μL ^―1 / 1.60 in .. mL ^―1获得了。这些最佳培养条件导致最大比生长为8.95％d ^-1（95％置信区间，8.73-9.18％d ^-1）和21.5％（95％置信区间，21.06-21.94％）的存活率，期望为高达99.6％。由于实际可行，建议将最佳三因子培养条件应用于实践，以使珍珠牡蛎P. martensii martensii幼虫martensii的生长和存活最大化。这项研究提供了宝贵的新见解，以提高珍珠牡蛎后期幼虫的生产效率。