The Yangtze River Delta White Goat is the only goat breed in the world that can produce superior-quality brush hair. Previous studies have shown that some genes are expressed differentially in the skin tissues between the goats produced superior-quality and normal-quality brush hair. Studies also have shown that different gene play varied roles in regulating the proliferation and apoptosis of hair follicle stem cells. However, the biological function of MAP3K1 (mitogen-activated protein kinase kinase kinase 1) gene in hair follicle stem cells is not fully understood. This study aims to investigate the role of MAP3K1 knockdown during the proliferation and apoptosis of hair follicle stem cells. RT-qPCR and Western blot were used to detect mRNA gene and protein expression level, CCK-8 and EdU assays were used to detect cell proliferation, and cell cycle and apoptosis were detected by flow cytometry. The results showed that the MAP3K1 expression level was significantly higher in the skin tissue of produced superior-quality brush hair than that in produced normal-quality brush hair. Moreover, functional studies indicated that si-MAP3K1 significantly inhibits the proliferation of hair follicle stem cells that came from a superior goat and promotes its apoptosis. Based on aforementioned assays, we speculated that MAP3K1 might play a regulatory effect in superior-quality brush hair traits.

长三角白山羊是世界上唯一可以生产优质刷毛的山羊品种。先前的研究表明，某些基因在山羊的皮肤组织中的表达存在差异，这些山羊的刷毛质量上等和质量正常。研究还表明，不同基因在调节毛囊干细胞的增殖和凋亡中发挥着不同的作用。然而，MAP3K1（丝裂原活化蛋白激酶激酶1）基因在毛囊干细胞中的生物学功能尚不完全清楚。本研究旨在探讨 MAP3K1 敲低在毛囊干细胞增殖和凋亡过程中的作用。RT-qPCR和Western blot检测mRNA基因和蛋白表达水平，CCK-8和EdU检测细胞增殖，流式细胞仪检测细胞周期和凋亡。结果表明，优质刷毛的皮肤组织中MAP3K1的表达水平明显高于正常刷毛的皮肤组织。此外，功能研究表明si-MAP3K1显着抑制来自优质山羊的毛囊干细胞的增殖并促进其凋亡。基于上述分析，我们推测 MAP3K1 可能在优质刷毛性状中发挥调节作用。功能研究表明，si-MAP3K1 显着抑制来自优质山羊的毛囊干细胞的增殖并促进其凋亡。基于上述分析，我们推测 MAP3K1 可能在优质刷毛性状中发挥调节作用。功能研究表明，si-MAP3K1 显着抑制来自优质山羊的毛囊干细胞的增殖并促进其凋亡。基于上述分析，我们推测 MAP3K1 可能在优质刷毛性状中发挥调节作用。