Abstract

Acid hydrolysis extensively applies as an economical pre-treatment to obtain monosaccharides from recalcitrant lignocellulosic residues. However, inhibitors are inevitably formed and reduce the downstream biofuel yield. Our bacterial isolate Burkholderia cepacia H-2 was capable of biotransforming 5-hydroxymethylfurfural (5-HMF), a common inhibitor, into valuable 2, 5-furan-dicarboxylic acid (FDCA). This isolate also has potential use for detoxifying other inhibitors derived from lignocellulosic acid hydrolysis. To integrate this strain into further lignocellulosic biofuel production processes, strain B. cepacia H-2 was immobilised to biotransform 5-HMF into FDCA and detoxify various inhibitors. Strain B. cepacia H-2 could biotransform 5-HMF into FDCA in the presence of inhibitors derived from lignocellulosic acid hydrolysis and detoxified formic acid, furfural and levulinic acid. The negative effect of approximately 500 mg L⁻¹ inhibitor on 5-HMF biotransformation was as follows (from strongest to weakest): furfural > acetic acid > phenol > formic acid > levulinic acid.

摘要

酸水解作为一种经济的预处理广泛应用于从顽固的木质纤维素残渣中获得单糖。然而，不可避免地会形成抑制剂并降低下游生物燃料的产量。我们的细菌分离株洋葱伯克霍尔德菌H-2 能够将常见的抑制剂 5-羟甲基糠醛 (5-HMF) 生物转化为有价值的 2, 5-呋喃二羧酸 (FDCA)。这种分离物还具有潜在的用途，可用于解毒源自木质纤维素酸水解的其他抑制剂。为了将该菌株整合到进一步的木质纤维素生物燃料生产过程中，将菌株B. cepacia H-2 固定化以将 5-HMF 生物转化为 FDCA 并解毒各种抑制剂。菌株B. cepacia在来自木质纤维素酸水解和解毒的甲酸、糠醛和乙酰丙酸的抑制剂存在下，H-2 可以将 5-HMF 生物转化为 FDCA。约500 mg L ^-1抑制剂对5-HMF生物转化的负面影响如下（从强到弱）：糠醛>乙酸>苯酚>甲酸>乙酰丙酸。