Purpose

To compare the bond strength of a universal adhesive system to bovine primary and human primary enamel and dentin.

Materials and Methods

Flat enamel and dentin surfaces from 28 sound human primary molars and 28 bovine primary incisors were bonded with Scotchbond Universal Adhesive applied in etch-and-rinse and self-etch mode (n=7). Composite resin cylinders (0.72 mm²) were built on the adhesive area. The specimens were submitted to a microshear bond strength test, and the bond failure mode was evaluated. Bond strength data (MPa) were analyzed using two-way ANOVA and Tukey´s test (α = 0.05). Energy-dispersive X-ray spectroscopy (EDS) was carried out to identify the levels of calcium and phosphorus in each type of teeth.

Results

Higher bond strength values were found to bovine primary enamel compared to human enamel (p = 0.04). No statistically significant differences were found between bovine and human primary dentin (p=0.09). The universal adhesive applied in self-etch mode to enamel resulted in lower bond strength values (p<0.05). EDS analysis showed significantly lower calcium content in bovine primary dentin (p=0.01), but no significant differences were found between bovine and human primary enamel.

Conclusion

Bovine primary teeth can only reliably replace human teeth in bonding studies when dentine is the focused substrate. Bovine primary enamel promotes higher bond strength values compared to human primary enamel.

中文翻译：

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通用粘合剂系统对牛和人乳牙的粘合强度

目的

比较通用粘合剂体系与牛初生和人类初生牙釉质和牙本质的粘合强度。

材料和方法

用Scotchbond通用胶粘剂将28个正常人的原牙和28个牛的门牙的牙釉质和牙本质平坦的表面粘合在一起，并以蚀刻，冲洗和自蚀刻的方式施加（n = 7）。复合树脂圆柱体（0.72 mm ²）建在粘合区域上。对样品进行微剪切粘结强度测试，并评估粘结破坏模式。使用双向方差分析和Tukey检验（α= 0.05）分析了粘结强度数据（MPa）。进行了能量色散X射线光谱（EDS），以鉴定每种类型牙齿中钙和磷的含量。

结果

发现与人类牙釉质相比，牛初级牙釉质的结合强度值更高（p = 0.04）。在牛和人原发性牙本质之间未发现统计学上的显着差异（p = 0.09）。以自蚀刻模式涂在瓷釉上的通用胶粘剂的粘结强度值较低（p <0.05）。EDS分析表明，牛初级牙本质中的钙含量明显降低（p = 0.01），但牛和人初级牙釉质之间没有发现显着差异。

结论

当以牙本质为主要基质时，在结合研究中，牛乳牙只能可靠地替代人的牙齿。与人的初级瓷釉相比，牛的初级瓷釉具有更高的粘结强度值。