Fumonisins are a group of mycotoxins commonly associated with corn-based products and require innovative alternatives to control exposure to its toxicity. The objective of this research was to determine the effect of amylose and resistant starch on fumonisin B1 (FB₁) levels in extruded corn-based products as well as the toxin bioaccessibility upon digestion. Cornmeal contaminated with FB₁ (1.5 µg/g) was extruded alone or combined with high-amylose corn starch (20%, w/w). FB₁ was quantified both in the unextruded and extruded products by HPLC (high-performance liquid chromatography) fluorescence detector with pre-column derivatization. Samples were then subjected to an in vitro digestion model to evaluate the stability of the interaction between FB₁ and the corn matrix extruded. The addition of high-amylose corn starch further reduced the detection of FB₁ (74.9%), when compared with the effect of the extrusion alone (66.0%), confirming the binding of FB₁ with the macromolecules or resistant starch. The bound fumonisin was stable upon simulated gastric digestion, and the duodenal bioaccessibility of free FB₁ was lower than 35% when high-amylose corn starch ingredient was used in the product. Principal component analysis (PCA) showed that high-amylose corn starch and resistant starch content influenced the reduction of FB₁ and its duodenal bioaccessibility. This study for the first time shows that addition of high-amylose corn starch during extrusion is an innovative strategy to reduce FB₁ release under digestive conditions, therefore useful in mitigating the exposure to this mycotoxin.

伏马菌素是一组通常与玉米产品相关的真菌毒素，需要创新的替代品来控制对其毒性的暴露。本研究的目的是确定直链淀粉和抗性淀粉对挤压出的基于玉米的产品中伏马菌素 B1 (FB ₁ ) 水平以及消化后毒素生物可及性的影响。被 FB ₁ (1.5 µg/g)污染的玉米粉单独或与高直链玉米淀粉 (20%, w / w )混合挤出。FB ₁通过带有柱前衍生化的 HPLC（高效液相色谱）荧光检测器对未挤出和挤出产品中的 然后对样品进行体外消化模型以评估 FB ₁和挤出的玉米基质之间相互作用的稳定性。与单独挤出的效果 (66.0%) 相比，添加高直链玉米淀粉进一步降低了 FB ₁的检测(74.9%)，证实了 FB ₁与大分子或抗性淀粉的结合。结合的伏马菌素在模拟胃消化时是稳定的，游离 FB ₁的十二指肠生物可及性当产品中使用高直链玉米淀粉成分时，其含量低于35%。主成分分析 (PCA) 表明，高直链玉米淀粉和抗性淀粉含量影响 FB ₁的减少及其十二指肠生物可及性。这项研究首次表明，在挤压过程中添加高直链玉米淀粉是减少消化条件下FB ₁释放的创新策略，因此有助于减少对这种霉菌毒素的暴露。